ELISA/assay

Mouse Cytochrome P450 2E1, CYP2E1 ELISA Kit

MBS933878

24-Strip-Wells (LIMIT 1)

240 USD

ELISA Kit

Mouse Cytochrome P450 2E1, CYP2E1 ELISA Kit

[cytochrome P450, family 2, subfamily E, polypeptide 1]

[Mouse Cytochrome P450 2E1 (CYP2E1) ELISA kit; CPE1; CYP2E; P450-J; P450C2E; cytochrome P450 2E1; cytochrome P450; subfamily IIE (ethanol-inducible); polypeptide 1; flavoprotein-linked monooxygenase; microsomal monooxygenase; xenobiotic monooxygenas; cytochrome P450; family 2; subfamily E; polypeptide 1]

[cytochrome P450 2E1; Cytochrome P450 2E1; cytochrome P450 2E1; CYPIIE1; cytochrome P450-J; cytochrome P450-ALC; 4-nitrophenol 2-hydroxylase; cytochrome P450, 2e1, ethanol inducible; cytochrome P450, family 2, subfamily e, polypeptide 1; 4-nitrophenol 2-hydroxylase (EC:1.14.13.n7); CYPIIE1; Cytochrome P450-ALC; Cytochrome P450-J]

[CYP2E1]

[Cyp2e1; Cyp2e1; Cyp2e; Cyp2e; Cyp2e-1]

CP2E1_MOUSE

Mouse

493

This assay has high sensitivity and excellent specificity for detection of human CYP2C9. No significant cross-reactivity or interference between human CYP2C9 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates. Assay Type: Quantitative Sandwich. Detection Range: 23.5 pg/ml-1500 pg/ml. Sensitivity: 5.8 pg/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CYP2C9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CYP2C9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYP2C9 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYP2C9 bound in the initial step. The color development is stopped and the intensity of the color is measured.

11276065

NP_067257.1

NM_021282.2

Q05421

56,805 Da

Arachidonic Acid Metabolism Pathway (83190); Arachidonic Acid Metabolism Pathway (366); Biological Oxidations Pathway (970688); CYP2E1 Reactions Pathway (970869); Chemical Carcinogenesis Pathway (673229); Chemical Carcinogenesis Pathway (673237); Cytochrome P450 Pathway (198390); Cytochrome P450 - Arranged By Substrate Type Pathway (971395); Drug Metabolism - Cytochrome P450 Pathway (83229); Drug Metabolism - Cytochrome P450 Pathway (427)

CYP2E1: Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms. Belongs to the cytochrome P450 family. Protein type: EC 1.14.13.n7; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - metabolism by cytochrome P450; Lipid Metabolism - linoleic acid; Endoplasmic reticulum; Lipid Metabolism - arachidonic acid; Oxidoreductase; Mitochondrial. Cellular Component: Golgi membrane; intrinsic to endoplasmic reticulum membrane; membrane; intracellular membrane-bound organelle; mitochondrion; endoplasmic reticulum. Molecular Function: 3-(3-hydroxy-phenyl)propionate hydroxylase activity; toluene-sulfonate methyl-monooxygenase activity; xylene monooxygenase activity; chlorophenol 4-monooxygenase activity; 6-hydroxy-3-methyl-2-oxo-1,2-dihydroquinoline 6-monooxygenase activity; metal ion binding; oxidoreductase activity; carbon disulfide oxygenase activity; toluene 4-monooxygenase activity; phenanthrene 9,10-monooxygenase activity; 1-hydroxy-2-oxolimonene 1,2-monooxygenase activity; 2-hydroxy-phenylacetate hydroxylase activity; 4-chlorobenzaldehyde oxidase activity; enzyme binding; 2-oxo-delta3-4,5,5-trimethylcyclopentenylacetyl-CoA 1,2-monooxygenase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; limonene monooxygenase activity; dibenzothiophene monooxygenase activity; phenanthrene 1,2-monooxygenase activity; 3,5-xylenol methylhydroxylase activity; 3-methyl-2-oxo-1,2-dihydroquinoline 6-monooxygenase activity; styrene monooxygenase activity; toluene 3-monooxygenase activity; monooxygenase activity; dimethyl sulfide monooxygenase activity; 4-hydroxyphenylacetate 3-monooxygenase activity; 4-nitrophenol 4-monooxygenase activity; 1-hydroxy-2-naphthoate hydroxylase activity; toluene-4-sulfonate monooxygenase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; tetrahydrofuran hydroxylase activity; oxygen binding; phenylacetate hydroxylase activity; toluene 2-monooxygenase activity; iron ion binding; 6-hydroxynicotinate 3-monooxygenase activity; heme binding; phenanthrene 3,4-monooxygenase activity; arachidonic acid epoxygenase activity; alpha-pinene monooxygenase [NADH] activity; steroid hydroxylase activity; 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase. Biological Process: steroid metabolic process; triacylglycerol metabolic process; xenobiotic metabolic process; exogenous drug catabolic process; monoterpenoid metabolic process; epoxygenase P450 pathway; heterocycle metabolic process; drug metabolic process

24-Strip-Wells (LIMIT 1)

240 USD

48-Strip-Wells

495

96-Strip-Wells

710

5x96-Strip-Wells

2705

10x96-Strip-Wells

5145