ELISA/assay

Human Ubiquitin-conjugating enzyme E2 T, UBE2T ELISA Kit

MBS9338696

48-Strip-Wells

470 USD

ELISA Kit

Human Ubiquitin-conjugating enzyme E2 T, UBE2T ELISA Kit

[ubiquitin-conjugating enzyme E2T (putative)]

[Human Ubiquitin-conjugating enzyme E2 T (UBE2T) ELISA kit; HSPC150; PIG50; HSPC150 protein similar to ubiquitin-conjugating enzyme; ubiquitin conjugating enzyme; ubiquitin-conjugating enzyme E2T; ubiquitin-conjugating enzyme E2T (putative)]

[ubiquitin-conjugating enzyme E2 T; Ubiquitin-conjugating enzyme E2 T; ubiquitin-conjugating enzyme E2 T; ubiquitin-protein ligase T; ubiquitin carrier protein T; ubiquitin conjugating enzyme; cell proliferation-inducing gene 50 protein; HSPC150 protein similar to ubiquitin-conjugating enzyme; ubiquitin-conjugating enzyme E2T (putative); Cell proliferation-inducing gene 50 protein; Ubiquitin carrier protein T; Ubiquitin-protein ligase T]

[UBE2T]

[UBE2T; UBE2T; PIG50; HSPC150; HSPC150; PIG50]

UBE2T_HUMAN

Human

No significant cross-reactivity or interference between this analyte and analogues is observed.

Store all reagents at 2-8 degree C

Assay Type: Quantitative Sandwich. Detection Range: 0.625 ng/ml - 20 ng/ml. Sensitivity: 0.1 ng/ml

Intra-assay Precision: Intra-assay CV(%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of UBE2T (hereafter termed "analyte") in undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.

7661808

NP_054895.1

NM_014176.3

Q9NPD8

22,521 Da

DNA Repair Pathway (105837); Fanconi Anemia Pathway (105895); Fanconi Anemia Pathway (377262); Fanconi Anemia Pathway (377128)

The covalent conjugation of ubiquitin to proteins regulates diverse cellular pathways and proteins. Ubiquitin is transferred to a target protein through a concerted action of a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), such as UBE2T, and a ubiquitin ligase (E3) (Machida et al., 2006 [PubMed 16916645]).[supplied by OMIM, Mar 2008]

UBE2T: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in mitomycin-C (MMC)-induced DNA repair: acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCL and FANCI. May contribute to ubiquitination and degradation of BRCA1. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer Lys-11 -, Lys-27 -, Lys-48 - and Lys-63 -linked polyubiquitination. Belongs to the ubiquitin-conjugating enzyme family. Protein type: Ubiquitin conjugating system; Ubiquitin ligase; EC 6.3.2.19; Ligase. Chromosomal Location of Human Ortholog: 1q32.1. Cellular Component: nucleoplasm; cytoplasm; nucleus. Molecular Function: small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; chromatin binding; ATP binding; ligase activity. Biological Process: protein monoubiquitination; protein autoubiquitination; DNA repair; response to DNA damage stimulus. Disease: Fanconi Anemia, Complementation Group T

48-Strip-Wells

470 USD

96-Strip-Wells

680

5x96-Strip-Wells

3100

10x96-Strip-Wells

6095