catalog number :
MBS9338610
products type :
ELISA Kit
products full name :
Human Ubl carboxyl-terminal hydrolase 18, USP18 ELISA Kit
products short name :
[ubiquitin specific peptidase 18]
products name syn :
[Human Ubl carboxyl-terminal hydrolase 18 (USP18) ELISA kit; ISG43; UBP43; ubiquitin specific protease 18; ubiquitin specific peptidase 18]
other names :
[ubl carboxyl-terminal hydrolase 18; Ubl carboxyl-terminal hydrolase 18; ubl carboxyl-terminal hydrolase 18; hUBP43; ubl thioesterase 18; ubl thiolesterase 18; 43 kDa ISG15-specific protease; ubiquitin specific protease 18; ISG15-specific-processing protease; ubiquitin specific peptidase 18; 43 kDa ISG15-specific protease; hUBP43; ISG15-specific-processing protease; Ubl thioesterase 18]
products gene name :
[USP18]
other gene names :
[USP18; USP18; ISG43; UBP43; ISG43; hUBP43]
uniprot entry name :
UBP18_HUMAN
specificity :
No significant cross-reactivity or interference between this analyte and analogues is observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Mouse Body Fluids, Tissue Homogenates, Secretions Or Feces Samples. Assay Type: Quantitative Sandwich. Detection Range: 0.625mmol/L-20mmol/L. Sensitivity: 0.1mmol/L.
other info2 :
Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
products description :
Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of UREA (hereafter termed "analyte") in undiluted original Mouse body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.
ncbi acc num :
NP_059110.2
ncbi gb acc num :
NM_017414.3
ncbi mol weight :
43,011 Da
ncbi pathways :
Antiviral Mechanism By IFN-stimulated Genes Pathway (530760); Cytokine Signaling In Immune System Pathway (366171); ISG15 Antiviral Mechanism Pathway (530761); Immune System Pathway (106386); Interferon Signaling Pathway (187103); Interferon Alpha/beta Signaling Pathway (187104); Regulation Of IFNA Signaling Pathway (187105)
ncbi summary :
The protein encoded by this gene belongs to the ubiquitin-specific proteases (UBP) family of enzymes that cleave ubiquitin from ubiquitinated protein substrates. It is highly expressed in liver and thymus, and is localized to the nucleus. This protein efficiently cleaves only ISG15 (a ubiquitin-like protein) fusions, and deletion of this gene in mice results in a massive increase of ISG15 conjugates in tissues, indicating that this protein is a major ISG15-specific protease. Mice lacking this gene are also hypersensitive to interferon, suggesting a function of this protein in downregulating interferon responses, independent of its isopeptidase activity towards ISG15. [provided by RefSeq, Sep 2011]
uniprot summary :
USP18: Can efficiently cleave only ISG15 fusions including native ISG15 conjugates linked via isopeptide bonds. Necessary to maintain a critical cellular balance of ISG15-conjugated proteins in both healthy and stressed organisms. Belongs to the peptidase C19 family. Protein type: Ubiquitin-specific protease; Protease; EC 3.4.19.-; EC 3.1.2.-. Chromosomal Location of Human Ortholog: 22q11.21. Cellular Component: nucleus; cytosol. Molecular Function: protein binding; ISG15-specific protease activity; cysteine-type endopeptidase activity; ubiquitin-specific protease activity. Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; cytokine and chemokine mediated signaling pathway
size4 :
10x96-Strip-Wells