product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Mouse Tryptophanyl-tRNA synthetase, cytoplasmic, WARS ELISA Kit
catalog :
MBS9338500
quantity :
48-Strip-Wells
price :
435 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS9338500
products type :
ELISA Kit
products full name :
Mouse Tryptophanyl-tRNA synthetase, cytoplasmic, WARS ELISA Kit
products short name :
tryptophanyl-tRNA synthetase
products name syn :
Mouse Tryptophanyl-tRNA synthetase; cytoplasmic (WARS) ELISA kit; GAMMA-2; IFI53; IFP53; interferon-induced protein 53; tryptophan tRNA ligase 1; cytoplasmic; tryptophanyl-tRNA synthetase
other names :
tryptophan--tRNA ligase, cytoplasmic isoform 2; Tryptophan--tRNA ligase, cytoplasmic; tryptophan--tRNA ligase, cytoplasmic; tryptophanyl-tRNA synthetase, cytoplasmic; tryptophanyl-tRNA synthetase; Tryptophanyl-tRNA synthetase; TrpRS
products gene name :
WARS
other gene names :
Wars; Wars; WRS; TrpRS; Wrs; TrpRS
uniprot entry name :
SYWC_MOUSE
reactivity :
Mouse
specificity :
No significant cross-reactivity or interference between Mouse WARS and analogues was observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 3.12 ng/ml - 100 ng/ml. Sensitivity: 1.0 ng/ml.
other info2 :
Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated WARS concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse WARS antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add WARS and WARS antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of WARS in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
products description :
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated WARS concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse WARS antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add WARS and WARS antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of WARS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi gi num :
256818806
ncbi acc num :
NP_001157786.1
ncbi gb acc num :
NM_001164314.1
uniprot acc num :
P32921
ncbi mol weight :
54,358 Da
ncbi pathways :
Aminoacyl-tRNA Biosynthesis Pathway (83227); Aminoacyl-tRNA Biosynthesis Pathway (424); Aminoacyl-tRNA Biosynthesis, Eukaryotes Pathway (421836); Aminoacyl-tRNA Biosynthesis, Eukaryotes Pathway (468360); Cytosolic TRNA Aminoacylation Pathway (574712); Gene Expression Pathway (574654); Tryptophan Metabolism Pathway (198332); TRNA Aminoacylation Pathway (574711)
uniprot summary :
WARS: Isoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression. Belongs to the class-I aminoacyl-tRNA synthetase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Ligase; Translation; EC 6.1.1.2; Amino Acid Metabolism - tryptophan. Cellular Component: cytoplasm; nucleus. Molecular Function: aminoacyl-tRNA ligase activity; tryptophan-tRNA ligase activity; nucleotide binding; ATP binding; ligase activity. Biological Process: tryptophanyl-tRNA aminoacylation; tRNA aminoacylation for protein translation; translation; angiogenesis; regulation of angiogenesis
size1 :
48-Strip-Wells
price1 :
435 USD
size2 :
96-Strip-Wells
price2 :
600
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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