Human Metastasis-suppressor KiSS-1, KISS1 ELISA Kit | MyBioSource MBS933835 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Metastasis-suppressor KiSS-1, KISS1 ELISA Kit

catalog :

MBS933835

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS933835

products type :

ELISA Kit

products full name :

Human Metastasis-suppressor KiSS-1, KISS1 ELISA Kit

products short name :

KiSS-1 metastasis-suppressor

products name syn :

Human Metastasis-suppressor KiSS-1 (KISS1) ELISA kit; KiSS-1; METASTIN; MGC39258; kisspeptin; malignant melanoma metastasis-suppressor; KiSS-1 metastasis-suppressor

other names :

metastasis-suppressor KiSS-1 preproprotein; Metastasis-suppressor KiSS-1; metastasis-suppressor KiSS-1; metastin; kisspeptin-1; prepro-kisspeptin; malignant melanoma metastasis-suppressor; KiSS-1 metastasis-suppressor; Kisspeptin-1Cleaved into the following 4 chains:MetastinAlternative name(s):Kisspeptin-54

products gene name :

KISS1

other gene names :

KISS1; KISS1; HH13; KiSS-1; PP5098

uniprot entry name :

KISS1_HUMAN

reactivity :

Human

sequence length :

138

specificity :

This assay has high sensitivity and excellent specificity for detection of human KISS1. No significant cross-reactivity or interference between human KISS1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma and tissue homogenates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml -20 ng/ml. Sensitivity: 0.078 ng/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for KISS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KISS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KISS1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KISS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

116829964

ncbi acc num :

NP_002247.3

ncbi gb acc num :

NM_002256.3

uniprot acc num :

Q15726

ncbi mol weight :

14,705 Da

ncbi pathways :

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Gastrin-CREB Signalling Pathway Via PKC And MAPK (645295); Peptide Ligand-binding Receptors Pathway (106358); Signal Transduction Pathway (477114); Signaling By GPCR Pathway (106356)

ncbi summary :

This gene is a metastasis suppressor gene that suppresses metastases of melanomas and breast carcinomas without affecting tumorigenicity. The encoded protein may inhibit chemotaxis and invasion and thereby attenuate metastasis in malignant melanomas. Studies suggest a putative role in the regulation of events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. A protein product of this gene, kisspeptin, stimulates gonadotropin-releasing hormone (GnRH)-induced gonadotropin secretion and regulates the pubertal activation of GnRH nuerons. A polymorphism in the terminal exon of this mRNA results in two protein isoforms. An adenosine present at the polymorphic site represents the third position in a stop codon. When the adenosine is absent, a downstream stop codon is utilized and the encoded protein extends for an additional seven amino acid residues. [provided by RefSeq, Mar 2012]

uniprot summary :

KiSS-1: Metastasis suppressor protein in malignant melanomas and in some breast cancers. May regulate events downstream of cell- matrix adhesion, perhaps involving cytoskeletal reorganization. Generates a C-terminally amidated peptide, metastin which functions as the endogenous ligand of the G-protein coupled receptor GPR54. Activation of the receptor inhibits cell proliferation and cell migration, key characteristics of tumor metastasis. Kp-10 is a decapeptide derived from the primary translation product, isolated in conditioned medium of first trimester trophoblast. Kp-10, but not other kisspeptins, increased intracellular Ca(2+) levels in isolated first trimester trophoblasts. Kp-10 is a paracrine/endocrine regulator in fine- tuning trophoblast invasion generated by the trophoblast itself. The receptor is also essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/GPR54 system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood. Belongs to the KISS1 family. Protein type: Secreted, signal peptide; Cell adhesion; Secreted. Chromosomal Location of Human Ortholog: 1q32. Cellular Component: extracellular region. Molecular Function: protein binding. Biological Process: cytoskeleton organization and biogenesis. Disease: Hypogonadotropic Hypogonadism 13 With Or Without Anosmia

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage