ELISA/assay

Rat Serine/threonine-protein kinase TNNI3K, TNNI3K ELISA Kit

MBS933726

48-Strip-Wells

510 USD

ELISA Kit

Rat Serine/threonine-protein kinase TNNI3K, TNNI3K ELISA Kit

TNNI3 interacting kinase

Rat Serine/threonine-protein kinase TNNI3K (TNNI3K) ELISA kit; CARK; MGC142099; MGC33828; cardiac ankyrin repeat kinase; TNNI3 interacting kinase

serine/threonine-protein kinase TNNI3K; Serine/threonine-protein kinase TNNI3K; serine/threonine-protein kinase TNNI3K; TNNI3-interacting kinase; cardiac ankyrin repeat kinase; TNNI3 interacting kinase; Cardiac ankyrin repeat kinase; TNNI3-interacting kinase

TNNI3K

Tnni3k; Tnni3k; Cark; Cark

TNI3K_RAT

Rat

835

This assay has high sensitivity and excellent specificity for detection of rat TNNI3K. No significant cross-reactivity or interference between rat TNNI3K and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 18.75 pg/ml-1200 pg/ml. Sensitivity: The minimum detectable dose of rat TNNI3K is typically less than 4.69 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TNNI3K has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNNI3K present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNNI3K is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNNI3K bound in the initial step. The color development is stopped and the intensity of the color is measured.

32401467

NP_861434.1

NM_181769.1

Q7TQP6

92,732 Da

Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)

kinase enzyme; interacts specifically with cardiac troponin I; human homolog is a cardiac specific kinase [RGD, Feb 2006]

TNNI3K: May play a role in cardiac physiology. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Protein kinase, TKL; EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); TKL group; MLK family; HH498 subfamily. Cellular Component: cytoplasm; nucleus. Molecular Function: protein C-terminus binding; protein serine/threonine kinase activity; troponin I binding; metal ion binding; ATP binding; protein kinase activity. Biological Process: regulation of heart rate; protein amino acid phosphorylation

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800