ELISA/assay

Mouse Superoxide dismutase [Mn], mitochondrial, SOD2 ELISA Kit

MBS933712

48-Strip-Wells

510 USD

ELISA Kit

Mouse Superoxide dismutase [Mn], mitochondrial, SOD2 ELISA Kit

superoxide dismutase 2, mitochondrial

Mouse Superoxide dismutase [Mn]; mitochondrial (SOD2) ELISA kit; IPO-B; MNSOD; MVCD6; Mn-SOD; Mn superoxide dismutase; indophenoloxidase B; manganese-containing superoxide dismutase; mangano-superoxide dismutase; superoxide dismutase 2; mitochondrial

superoxide dismutase; Superoxide dismutase [Mn], mitochondrial; superoxide dismutase [Mn], mitochondrial; superoxide dismutase [Mn], mitochondrial; manganese SOD; manganese superoxide dismutase; superoxide dismutase 2, mitochondrial

SOD2

Sod2; Sod2; MnSOD; Sod-2; Sod-2

SODM_MOUSE

Mouse

222

This assay has high sensitivity and excellent specificity for detection of Mouse SOD2. No significant cross-reactivity or interference between Mouse SOD2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.625 ng/ml-40 ng/ml. Sensitivity: 0.156 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SOD2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SOD2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SOD2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SOD2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

31980762

NP_038699.2

NM_013671.3

P09671

24,603 Da

Cellular Responses To Stress Pathway (971022); Detoxification Of Reactive Oxygen Species Pathway (970575); FoxO Signaling Pathway (921412); Huntington's Disease Pathway (83297); Huntington's Disease Pathway (512); One Carbon Metabolism And Related Pathways (198380); Oxidative Stress Pathway (198384); Peroxisome Pathway (131230); Peroxisome Pathway (131126); Superoxide Radicals Degradation Pathway (143050)

SOD2: Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems. Genetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new- onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Belongs to the iron/manganese superoxide dismutase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 1.15.1.1; Oxidoreductase; Mitochondrial. Cellular Component: mitochondrion; cytoplasm; mitochondrial inner membrane; intracellular; myelin sheath. Molecular Function: identical protein binding; protein binding; DNA binding; manganese ion binding; metal ion binding; superoxide dismutase activity; oxidoreductase activity; oxygen binding. Biological Process: oxygen homeostasis; positive regulation of nitric oxide biosynthetic process; removal of superoxide radicals; heart development; locomotory behavior; vasodilation; post-embryonic development; age-dependent response to oxidative stress; protein homotetramerization; negative regulation of cell proliferation; apoptotic mitochondrial changes; glutathione metabolic process; sensory perception of sound; regulation of mitochondrial membrane potential; acetylcholine vasodilation involved in regulation of systemic arterial blood pressure; regulation of catalytic activity; regulation of blood pressure; response to gamma radiation; hemopoiesis; response to axon injury; negative regulation of neuron apoptosis; protein homooligomerization; response to drug; response to nutrient levels; erythrophore differentiation; mitochondrion organization and biogenesis; release of cytochrome c from mitochondria; response to superoxide; superoxide metabolic process; liver development; negative regulation of fat cell differentiation; regulation of transcription from RNA polymerase II promoter; iron ion homeostasis; response to reactive oxygen species; response to hyperoxia; DNA damage response, signal transduction resulting in induction of apoptosis; response to hydrogen peroxide; age-dependent response to reactive oxygen species; negative regulation of fibroblast proliferation; detection of oxygen; hydrogen peroxide metabolic process; neuron development; response to oxidative stress; response to activity; induction of apoptosis by oxidative stress; superoxide release; hydrogen peroxide biosynthetic process; negative regulation of apoptosis

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800