Human P-selectin glycoprotein ligand 1, SELPLG ELISA Kit | MyBioSource MBS933692 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human P-selectin glycoprotein ligand 1, SELPLG ELISA Kit

catalog :

MBS933692

quantity :

96 Strip Wells

price :

760 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS933692

products type :

ELISA Kit

products full name :

Human P-selectin glycoprotein ligand 1, SELPLG ELISA Kit

products short name :

selectin P ligand

products name syn :

Human P-selectin glycoprotein ligand 1 (SELPLG) ELISA kit; CD162; CLA; PSGL-1; PSGL1; cutaneous lymphocyte-associated associated antigen; selectin P ligand

other names :

P-selectin glycoprotein ligand 1 isoform 1; P-selectin glycoprotein ligand 1; P-selectin glycoprotein ligand 1; cutaneous lymphocyte-associated associated antigen; selectin P ligand; Selectin P ligand; CD_antigen: CD162

products gene name :

SELPLG

other gene names :

SELPLG; SELPLG; CLA; CD162; PSGL1; PSGL-1; PSGL-1

uniprot entry name :

SELPL_HUMAN

reactivity :

Human

sequence length :

412

specificity :

This assay has high sensitivity and excellent specificity for detection of human SELPLG. No significant cross-reactivity or interference between human SELPLG and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: For the quantitative determination of human P-selectin glycoprotein ligand 1 (SELPLG) concentrations in serum, plasma, tissue homogenates. Detection Range: 7.8 U/ml -500 U/ml.

other info2 :

Sensitivity: The minimum detectable dose of human SELPLG is typically less than 1.95 U/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SELPLG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SELPLG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SELPLG is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SELPLG bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

331284238

ncbi acc num :

NP_001193538.1

ncbi gb acc num :

NM_001206609.1

uniprot acc num :

Q14242

ncbi mol weight :

43,201 Da

ncbi pathways :

Cell Adhesion Molecules (CAMs) Pathway 83069!!Cell Adhesion Molecules (CAMs) Pathway 480!!Cell Surface Interactions At The Vascular Wall Pathway 106062!!Hemostasis Pathway 106028!!Staphylococcus Aureus Infection Pathway 172846!!Staphylococcus Aureus Infection Pathway 171867!!Amb2 Integrin Signaling Pathway 137945

ncbi summary :

This gene encodes a glycoprotein that functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2011]

uniprot summary :

Function: A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.2 PublicationsManual assertion based on experiment in:Ref.13

size :

96 Strip Wells

price :

760 USD

more info or order :

MyBioSource product webpage