catalog number :
MBS9336878
products type :
ELISA Kit
products full name :
Human NEDD8-activating enzyme E1 regulatory subunit, NAE1 ELISA Kit
products short name :
[NEDD8 activating enzyme E1 subunit 1]
products name syn :
[Human NEDD8-activating enzyme E1 regulatory subunit (NAE1) ELISA kit; A-116A10.1; APPBP1; HPP1; ula-1; NEDD8-activating enzyme E1 subunit; amyloid beta precursor protein binding protein 1; 59kDa; amyloid beta precursor protein-binding protein 1; 59kD; amyloid protein-bin; NEDD8 activating enzyme E1 subunit 1]
other names :
[NEDD8-activating enzyme E1 regulatory subunit isoform b; NEDD8-activating enzyme E1 regulatory subunit; NEDD8-activating enzyme E1 regulatory subunit; APP-BP1; protooncogene protein 1; proto-oncogene protein 1; amyloid protein-binding protein 1; NEDD8-activating enzyme E1 subunit; amyloid beta precursor protein-binding protein 1, 59kD; amyloid beta precursor protein binding protein 1, 59kDa; amyloid beta precursor protein-binding protein 1, 59 kDa; NEDD8 activating enzyme E1 subunit 1; Amyloid beta precursor protein-binding protein 1, 59 kDa; APP-BP1; Amyloid protein-binding protein 1; Proto-oncogene protein 1]
products gene name :
[NAE1]
other gene names :
[NAE1; NAE1; HPP1; ula-1; APPBP1; A-116A10.1; APPBP1; HPP1; APP-BP1]
uniprot entry name :
ULA1_HUMAN
specificity :
No significant cross-reactivity or interference between this analyte and analogues is observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Assay Type: Quantitative Sandwich. Detection Range: 0.625 ng/ml - 20 ng/ml. Sensitivity: 0.1 ng/ml.
other info2 :
Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
products description :
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of NAE1 (hereafter termed "analyte") in undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.
ncbi acc num :
NP_001018169.1
ncbi gb acc num :
NM_001018159.1
ncbi mol weight :
60,246 Da
ncbi pathways :
Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509)
ncbi summary :
The protein encoded by this gene binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. In addition, the encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. This protein is required for cell cycle progression through the S/M checkpoint. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
uniprot summary :
APPBP1: Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation. Belongs to the ubiquitin-activating E1 family. ULA1 subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Ubiquitin conjugating system. Chromosomal Location of Human Ortholog: 16q22. Cellular Component: cytoplasm; plasma membrane; cytosol; nucleus. Molecular Function: NEDD8 activating enzyme activity; protein binding; protein heterodimerization activity; ubiquitin protein ligase binding. Biological Process: regulation of apoptosis; protein neddylation; neuron apoptosis; regulation of neuron apoptosis; mitotic cell cycle DNA replication checkpoint; signal transduction
size4 :
10x96-Strip-Wells