catalog number :
MBS933681
products type :
ELISA Kit
products full name :
Human T-cell antigen CD7, CD7 ELISA Kit
products short name :
CD7 molecule
products name syn :
Human T-cell antigen CD7 (CD7) ELISA kit; GP40; LEU-9; TP41; Tp40; CD7 antigen; CD7 antigen (p41) ; T-cell antigen CD7; T-cell leukemia antigen; p41 protein; CD7 molecule
other names :
T-cell antigen CD7; T-cell antigen CD7; T-cell antigen CD7; p41 protein; CD7 antigen (p41); T-cell leukemia antigen; T-cell surface antigen Leu-9; CD7 molecule; GP40; T-cell leukemia antigen; T-cell surface antigen Leu-9; TP41; CD_antigen: CD7
other gene names :
CD7; CD7; GP40; TP41; Tp40; LEU-9
uniprot entry name :
CD7_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human CD7. No significant cross-reactivity or interference between human CD7 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: The minimum detectable dose of human CD7 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CD7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD7 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_006128.1
ncbi gb acc num :
NM_006137.6
ncbi mol weight :
25,409 Da
ncbi pathways :
Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489)
ncbi summary :
This gene encodes a transmembrane protein which is a member of the immunoglobulin superfamily. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development. [provided by RefSeq, Jul 2008]
uniprot summary :
CD7: Not yet known. Protein type: Membrane protein, integral; Immunoglobulin superfamily. Chromosomal Location of Human Ortholog: 17q25.2-q25.3. Cellular Component: membrane; plasma membrane; integral to membrane. Molecular Function: receptor activity. Biological Process: T cell activation; homeostasis of number of cells within a tissue; immune response; transmembrane receptor protein tyrosine kinase signaling pathway
size4 :
10x96-Strip-Wells