Mouse Elongation factor 1-alpha 1, EEF1A1 ELISA Kit | MyBioSource MBS9336767 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Elongation factor 1-alpha 1, EEF1A1 ELISA Kit

catalog :

MBS9336767

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS9336767

products type :

ELISA Kit

products full name :

Mouse Elongation factor 1-alpha 1, EEF1A1 ELISA Kit

products short name :

eukaryotic translation elongation factor 1 alpha 1

products name syn :

Mouse Elongation factor 1-alpha 1 (EEF1A1) ELISA kit; RP11-505P4.2; CCS-3; CCS3; EEF-1; EEF1A; EF-Tu; EF1A; FLJ25721; GRAF-1EF; HNGC:16303; LENG7; MGC102687; MGC131894; MGC16224; PTI1; eEF1A-1; CTCL tumor antigen; EF1a-like protein; OTTHUMP00000063804; ce; eukaryotic translation elongation factor 1 alpha 1

other names :

elongation factor 1-alpha 1; Elongation factor 1-alpha 1; elongation factor 1-alpha 1; EF-Tu; eEF1A-1; EF-1-alpha-1; elongation factor Tu; elongation factor 1 A-1; eukaryotic elongation factor 1 A-1; eukaryotic translation elongation factor 1 alpha 1; Elongation factor Tu; EF-Tu; Eukaryotic elongation factor 1 A-1; eEF1A-1

products gene name :

EEF1A1

other gene names :

Eef1a1; Eef1a1; Eef1a; EF-1-alpha-1; EF-Tu; eEF1A-1

uniprot entry name :

EF1A1_MOUSE

reactivity :

Mouse

specificity :

No significant cross-reactivity or interference between Mouse EEF1A1 and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 0.25 ng/ml - 8 ng/ml. Sensitivity: 0.1 ng/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated EEF1A1 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse EEF1A1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add EEF1A1 and EEF1A1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of EEF1A1 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated EEF1A1 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse EEF1A1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add EEF1A1 and EEF1A1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of EEF1A1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

126032329

ncbi acc num :

NP_034236.2

ncbi gb acc num :

NM_010106.2

uniprot acc num :

P10126

ncbi mol weight :

50,114 Da

ncbi pathways :

EGFR1 Signaling Pathway (198278); Eukaryotic Translation Elongation Pathway (574734); Gene Expression Pathway (574654); Legionellosis Pathway (469202); Legionellosis Pathway (469186); Metabolism Of Proteins Pathway (575185); Peptide Chain Elongation Pathway (574735); RNA Transport Pathway (177887); RNA Transport Pathway (175229); Translation Pathway (574722)

uniprot summary :

EEF1A1: an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. Promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Protein type: Translation; Translation elongation; Nucleolus. Cellular Component: extracellular space; cortical actin cytoskeleton; membrane; cytoplasm; plasma membrane; nucleolus; cytosol; nucleus; myelin sheath. Molecular Function: mRNA binding; GTPase activity; protein binding; GTP binding; nucleotide binding; protein kinase binding; tRNA binding; translation elongation factor activity. Biological Process: translational elongation; translation; protein homooligomerization

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage