ELISA/assay

Mouse G protein-coupled receptor kinase 5, GRK5 ELISA Kit

MBS9336679

48-Strip-Wells

435 USD

ELISA Kit

Mouse G protein-coupled receptor kinase 5, GRK5 ELISA Kit

G protein-coupled receptor kinase 5

Mouse G protein-coupled receptor kinase 5 (GRK5) ELISA kit; GPRK5; ; G protein-coupled receptor kinase 5

G protein-coupled receptor kinase 5; G protein-coupled receptor kinase 5; G protein-coupled receptor kinase 5; g protein-coupled receptor kinase GRK5; G protein-coupled receptor kinase 5; G protein-coupled receptor kinase GRK5

GRK5

Grk5; Grk5; Gprk5; Gprk5

GRK5_MOUSE

Mouse

No significant cross-reactivity or interference between Mouse GRK5 and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 62.5 pg/ml - 2000 pg/ml. Sensitivity: 10 pg/ml.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated GRK5 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse GRK5 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GRK5 and GRK5 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GRK5 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated GRK5 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse GRK5 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GRK5 and GRK5 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GRK5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

159032059

NP_061357.3

NM_018869.3

Q8VEB1

67,732 Da

Calcium Regulation In The Cardiac Cell Pathway (198327); Chemokine Signaling Pathway (99271); Chemokine Signaling Pathway (96864); Endocytosis Pathway (102286); Endocytosis Pathway (102181); G Alpha (q) Signalling Events Pathway (575456); GPCR Downstream Signaling Pathway (575452); Morphine Addiction Pathway (552689); Morphine Addiction Pathway (554808); Myometrial Relaxation And Contraction Pathways (198333)

GRK5: Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein- coupled receptors (GPCRs). Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their down-regulation. Phosphorylates a variety of GPCRs, including adrenergic receptors, muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4 subtypes), dopamine receptors and opioid receptors. In addition to GPCRs, also phosphorylates various substrates: Hsc70- interacting protein/ST13, TP53/p53, HDAC5, and arrestin-1/ARRB1. Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. Phosphorylation of HDAC5, a repressor of myocyte enhancer factor 2 (MEF2) leading to nuclear export of HDAC5 and allowing MEF2- mediated transcription. Phosphorylation of TP53/p53, a crucial tumor suppressor, inhibits TP53/p53-mediated apoptosis. Phosphorylation of ST13 regulates internalization of the chemokine receptor. Phosphorylates rhodopsin (RHO) (in vitro) and a non G- protein-coupled receptor, LRP6 during Wnt signaling (in vitro). Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.16; Kinase, protein; Protein kinase, AGC; AGC group; GRK family; GRK subfamily. Cellular Component: nucleoplasm; nuclear membrane; focal adhesion; membrane; cytoplasm; plasma membrane; nucleus. Molecular Function: transferase activity; protein serine/threonine kinase activity; G-protein coupled receptor kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; lipid binding; ATP binding; protein kinase activity. Biological Process: desensitization of G-protein coupled receptor protein signaling pathway; tachykinin signaling pathway; Wnt receptor signaling pathway; apoptosis; protein amino acid autophosphorylation; signal transduction; phosphorylation; protein amino acid phosphorylation; regulation of G-protein coupled receptor protein signaling pathway; negative regulation of apoptosis

48-Strip-Wells

435 USD

96-Strip-Wells

600