Human E3 ubiquitin-protein ligase SMURF2, SMURF2 ELISA Kit | MyBioSource MBS9336626 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human E3 ubiquitin-protein ligase SMURF2, SMURF2 ELISA Kit

catalog :

MBS9336626

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS9336626

products type :

ELISA Kit

products full name :

Human E3 ubiquitin-protein ligase SMURF2, SMURF2 ELISA Kit

products short name :

[SMAD specific E3 ubiquitin protein ligase 2]

products name syn :

[Human E3 ubiquitin-protein ligase SMURF2 (SMURF2) ELISA kit; DKFZp686F0270; MGC138150; E3 ubiquitin ligase SMURF2; SMAD specific E3 ubiquitin protein ligase 2]

other names :

[E3 ubiquitin-protein ligase SMURF2; E3 ubiquitin-protein ligase SMURF2; E3 ubiquitin-protein ligase SMURF2; hSMURF2; E3 ubiquitin ligase SMURF2; SMAD ubiquitination regulatory factor 2; SMAD-specific E3 ubiquitin-protein ligase 2; SMAD specific E3 ubiquitin protein ligase 2; SMAD ubiquitination regulatory factor 2; SMAD-specific E3 ubiquitin-protein ligase 2]

products gene name :

[SMURF2]

other gene names :

[SMURF2; SMURF2; hSMURF2]

uniprot entry name :

SMUF2_HUMAN

reactivity :

Human

specificity :

No significant cross-reactivity or interference between this analyte and analogues is observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Assay Type: Quantitative Sandwich. Detection Range: 1.56 ng/ml - 50 ng/ml. Sensitivity: 0.1 ng/ml

other info2 :

Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of SMURF2 (hereafter termed "analyte") in undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.

ncbi gi num :

12232397

ncbi acc num :

NP_073576.1

ncbi gb acc num :

NM_022739.3

uniprot acc num :

Q9HAU4

ncbi mol weight :

86,196 Da

ncbi pathways :

Adaptive Immune System Pathway (366160); Antigen Processing: Ubiquitination Proteasome Degradation Pathway (366162); BMP Receptor Signaling Pathway (137948); Class I MHC Mediated Antigen Processing Presentation Pathway (366161); Endocytosis Pathway (102279); Endocytosis Pathway (102181); Immune System Pathway (106386); Signal Transduction Pathway (477114); Signaling By BMP Pathway (106336); Signaling By TGF Beta Pathway (106504)

uniprot summary :

SMURF2: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level. Protein type: EC 6.3.2.19; Ubiquitin conjugating system; Ubiquitin ligase; EC 6.3.2.-; Ligase. Chromosomal Location of Human Ortholog: 17q22-q23. Cellular Component: nucleoplasm; cytoplasm; plasma membrane; cytosol; nucleus; ubiquitin ligase complex; lipid raft. Molecular Function: identical protein binding; protein binding; ubiquitin-protein ligase activity; SMAD binding; transforming growth factor beta receptor binding; ligase activity. Biological Process: BMP signaling pathway; ubiquitin-dependent protein catabolic process; transcription initiation from RNA polymerase II promoter; regulation of transforming growth factor beta receptor signaling pathway; transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; protein ubiquitination during ubiquitin-dependent protein catabolic process; gene expression; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of transforming growth factor beta receptor signaling pathway; ubiquitin-dependent SMAD protein catabolic process

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

680

size3 :

5x96-Strip-Wells

price3 :

3100

size4 :

10x96-Strip-Wells

price4 :

6095

more info or order :

MyBioSource product webpage