Mouse Disks large homolog 1, DLG1 ELISA Kit | MyBioSource MBS9336613 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Disks large homolog 1, DLG1 ELISA Kit

catalog :

MBS9336613

quantity :

48-Strip-Wells

price :

470 USD

product information

catalog number :

MBS9336613

products type :

ELISA Kit

products full name :

Mouse Disks large homolog 1, DLG1 ELISA Kit

products short name :

[discs, large homolog 1 (Drosophila)]

products name syn :

[Mouse Disks large homolog 1 (DLG1) ELISA kit; DKFZp761P0818; DKFZp781B0426; DLGH1; SAP-97; SAP97; dJ1061C18.1.1; hdlg; OTTHUMP00000165203; discs large homolog 1; discs; large homolog 1; presynaptic protein SAP97; synapse-associated protein 97; discs; large homolog 1 (Drosophila)]

other names :

[disks large homolog 1 isoform 2; Disks large homolog 1; disks large homolog 1; embryo-dlg/synapse-associated protein 97; discs, large homolog 1 (Drosophila); Embryo-dlg/synapse-associated protein 97; E-dlg/SAP97; Synapse-associated protein 97; SAP-97; SAP97]

products gene name :

[DLG1]

other gene names :

[Dlg1; Dlg1; Dlgh1; SAP97; SAP-97; mKIAA4187; E-dlg/SAP97; B130052P05Rik; Dlgh1; E-dlg/SAP97; SAP-97; SAP97]

uniprot entry name :

DLG1_MOUSE

reactivity :

Mouse

specificity :

No significant cross-reactivity or interference between Mouse DLG1 and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 0.625 ng/ml - 20 ng/ml. Sensitivity: 0.1 ng/ml.

other info2 :

Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].

products description :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated DLG1 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse DLG1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add DLG1 and DLG1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of DLG1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

356995917

ncbi acc num :

NP_001239362.1

ncbi gb acc num :

NM_001252433.1

uniprot acc num :

Q811D0

ncbi mol weight :

100,120 Da

ncbi pathways :

Axon Guidance Pathway (575211); Developmental Biology Pathway (575210); Glutamate Binding, Activation Of AMPA Receptors And Synaptic Plasticity Pathway (575076); HTLV-I Infection Pathway (373903); HTLV-I Infection Pathway (373889); L1CAM Interactions Pathway (575231); Neuronal System Pathway (575054); Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell Pathway (575075); NrCAM Interactions Pathway (575236); T Cell Receptor Signaling Pathway (198362)

uniprot summary :

DLG1: the human homologue of the Drosophila discs large tumor suppressor is an essential multidomain scaffolding protein required for normal development. Recruits channels, receptors and signaling molecules to discrete plasma membrane domains in polarized cells. May play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Interacts through its guanylate kinase-like domain with DLGAP1/2/3/4, and MAP1A. May interact with PTEN, HTR2A and LRFN1. Interacts through its PDZ domains with NMDAR2A, KV1.1, KV1.2, KV1.3, KV1.4, KV1.5, GLUR1, GPR124 and GPR125. Interacts with cytoskeleton-associated proteins EPB41 and EZR. Found in a complex with KV1.5 and CAV3. Found in a complex with APC and CTNNB1. Interacts with CDH1 through binding to PIK3R1. Forms multiprotein complexes with CASK, LIN7A, LIN7B, LIN7C, APBA1, and KCNJ12. Interacts through its guanylate kinase-like domain with KIF13B. Forms a tripartite complex composed of DLG1, MPP7 and LIN7A/C. May interact with TJAP1. Interacts with TOPK and the HTLV-1 viral Tax and HPV-18 E6 papillomavirus (HPV) oncoproteins. The alternatively spliced domain I3 corresponding to amino acids (636-669) of isoform 4 is an EPB41 binding site mediating association to membranes in polarized and non-polarized cells. The PDZ domains may also mediate association to membranes by binding to EPB41 and GPR124 together with the L27 domain that binds CASK and DLG2. The L27 domain may regulate DLG1 self-association. The N-terminal alternatively spliced region is capable of binding several SH3 domains and also moderates the level of protein oligomerization. Processes such as wound healing and tissue maintenance dictate that cells maintain dynamic control of junction assembly and disassembly. Many pathologic processes such as cancer and invasion of host cells by microbes affect these pathways. The HPV-18 E6 oncogene sequesters INADL and its paralog MPDZ as well as DLG1 and targets them for degradation, contributing to loss of polarity. Seven human isoforms produced by alternative splicing have been described. Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold. Cellular Component: Golgi apparatus; microtubule; basolateral plasma membrane; T-tubule; immunological synapse; intercellular junction; lipid raft; postsynaptic membrane; cell-cell adherens junction; membrane; perinuclear region of cytoplasm; cell projection membrane; cytoplasm; basal plasma membrane; synapse; basal lamina; neuromuscular junction; lateral plasma membrane; neuron projection; internal side of plasma membrane; tight junction; endoplasmic reticulum; lateral loop; postsynaptic density; presynaptic membrane; plasma membrane; ionotropic glutamate receptor complex; cell junction; nucleus. Molecular Function: protein C-terminus binding; guanylate kinase activity; ionotropic glutamate receptor binding; protein binding; potassium channel regulator activity; mitogen-activated protein kinase kinase binding; protein complex scaffold; kinase binding; protein kinase binding; phosphatase binding; PDZ domain binding. Biological Process: lens development in camera-type eye; peristalsis; regulation of myelination; formation of immunological synapse; T cell activation; positive regulation of potassium ion transport; positive regulation of multicellular organism growth; receptor clustering; smooth muscle development; synaptic transmission; cell-cell adhesion; protein localization; regulation of protein localization; negative regulation of T cell proliferation; T cell cytokine production; ureteric bud development; positive regulation of cell proliferation; hard palate development; negative regulation of mitotic cell cycle; cortical actin cytoskeleton organization and biogenesis; negative regulation of epithelial cell proliferation; nucleotide phosphorylation; positive regulation of developmental growth; tissue morphogenesis; negative regulation of protein kinase B signaling cascade; actin filament organization; embryonic skeletal morphogenesis; establishment and/or maintenance of epithelial cell polarity; regulation of membrane potential; positive regulation of actin filament polymerization; ureteric bud branching; amyloid precursor protein metabolic process; endothelial cell proliferation; activation of protein kinase activity; lipid raft organization and biogenesis; reproductive structure development

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

680

size3 :

5x96-Strip-Wells

price3 :

3100

size4 :

10x96-Strip-Wells

price4 :

6095