ELISA/assay

Rat Gamma-aminobutyric acid receptor subunit gamma-2, GABRG2 ELISA Kit

MBS9336526

48-Strip-Wells

435 USD

ELISA Kit

Rat Gamma-aminobutyric acid receptor subunit gamma-2, GABRG2 ELISA Kit

gamma-aminobutyric acid (GABA) A receptor, gamma 2

Rat Gamma-aminobutyric acid receptor subunit gamma-2 (GABRG2) ELISA kit; CAE2; ECA2; GEFSP3; gamma-aminobutyric acid A receptor; gamma 2; gamma-aminobutyric acid (GABA) A receptor; gamma 2

gamma-aminobutyric acid receptor subunit gamma-2; Gamma-aminobutyric acid receptor subunit gamma-2; gamma-aminobutyric acid receptor subunit gamma-2; GABA(A) receptor subunit gamma-2; gamma-aminobutyric acid A receptor, gamma 2; gamma-aminobutyric acid A receptor, gamma 2; gamma-aminobutyric acid (GABA-A) receptor, subunit gamma 2; gamma-aminobutyric acid (GABA) A receptor, gamma 2; GABA(A) receptor subunit gamma-2

GABRG2

Gabrg2; Gabrg2

GBRG2_RAT

Rat

No significant cross-reactivity or interference between Rat GABRG2 and analogues was observed.

Store all reagents at 2-8 degree C

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 0.25 umol/L - 8 umol/L. Sensitivity: 0.1 umol/L.

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated GABRG2 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat GABRG2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GABRG2 and GABRG2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GABRG2 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated GABRG2 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat GABRG2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GABRG2 and GABRG2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GABRG2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

34328527

NP_899156.1

NM_183327.1

P18508

54,077 Da

GABAergic Synapse Pathway (377244); GABAergic Synapse Pathway (377129); Morphine Addiction Pathway (552694); Morphine Addiction Pathway (554808); Neuroactive Ligand-receptor Interaction Pathway (83445); Neuroactive Ligand-receptor Interaction Pathway (462); Nicotine Addiction Pathway (583273); Nicotine Addiction Pathway (584676); Retrograde Endocannabinoid Signaling Pathway (537882); Retrograde Endocannabinoid Signaling Pathway (539806)

subunit of GABA receptors, which bind benzodiazepines and act as inhibitory ion channels in the brain [RGD, Feb 2006]

GABRG2 iso2: GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. Defects in GABRG2 are the cause of childhood absence epilepsy type 2 (ECA2). ECA2 is a subtype of idiopathic generalized epilepsy (IGE) characterized by an onset at age 6-7 years, frequent absence seizures (several per day) and bilateral, synchronous, symmetric 3-Hz spike waves on EEG. During adolescence, tonic-clonic and myoclonic seizures develop. Some individuals manifest ECA2 occurring in combination with febrile convulsions. Defects in GABRG2 are the cause of familial febrile convulsions type 8 (FEB8). A febrile convulsion is defined as a seizure event in infancy or childhood, usually occurring between 6 months and 6 years of age, associated with fever but without any evidence of intracranial infection or defined pathologic or traumatic cause. Febrile convulsions affect 5-12% of infants and children up to 6 years of age. There is epidemiological evidence that febrile seizures are associated with subsequent afebrile and unprovoked seizures in 2% to 7% of patients. Defects in GABRG2 are the cause of generalized epilepsy with febrile seizures plus type 3 (GEFS+3). Generalized epilepsy with febrile seizures-plus refers to a rare autosomal dominant, familial condition with incomplete penetrance and large intrafamilial variability. Patients display febrile seizures persisting sometimes beyond the age of 6 years and/or a variety of afebrile seizure types. GEFS+ is a disease combining febrile seizures, generalized seizures often precipitated by fever at age 6 years or more, and partial seizures, with a variable degree of severity. Defects in GABRG2 are a cause of severe myoclonic epilepsy in infancy (SMEI); also called Dravet syndrome. SMEI is a rare disorder characterized by generalized tonic, clonic, and tonic-clonic seizures that are initially induced by fever and begin during the first year of life. Later, patients also manifest other seizure types, including absence, myoclonic, and simple and complex partial seizures. Psychomotor development delay is observed around the second year of life. SMEI is considered to be the most severe phenotype within the spectrum of generalized epilepsies with febrile seizures-plus. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRG2 sub-subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Channel, chloride; Membrane protein, multi-pass; Membrane protein, integral; Transporter, ion channel; Transporter; Channel, ligand-gated. Cellular Component: postsynaptic membrane; axon; integral to plasma membrane; cytoplasm; cell junction. Molecular Function: chloride channel activity; protein binding; GABA-A receptor activity; GABA receptor activity; inhibitory extracellular ligand-gated ion channel activity. Biological Process: synaptic transmission; adult behavior; synaptic transmission, GABAergic; chloride transport; signal transduction; gamma-aminobutyric acid signaling pathway; post-embryonic development

48-Strip-Wells

435 USD

96-Strip-Wells

600