Mouse R-spondin-1, RSPO1 ELISA Kit | MyBioSource MBS933642 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse R-spondin-1, RSPO1 ELISA Kit

catalog :

MBS933642

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS933642

products type :

ELISA Kit

products full name :

Mouse R-spondin-1, RSPO1 ELISA Kit

products short name :

R-spondin homolog (Xenopus laevis)

products name syn :

Mouse R-spondin-1 (RSPO1) ELISA kit; RP11-566C13.1; CRISTIN3; FLJ40906; RSPO; OTTHUMP00000180612; OTTHUMP00000180613; R-spondin1; roof plate-specific spondin; R-spondin homolog (Xenopus laevis)

other names :

R-spondin-1; R-spondin-1; R-spondin-1; cristin-3; mCristin-3; roof plate-specific spondin-1; thrombospondin type 1 domain containing gene Rspondin; cysteine-rich and single thrombospondin domain-containing protein 3; R-spondin homolog (Xenopus laevis); Cysteine-rich and single thrombospondin domain-containing protein 3; Cristin-3; mCristin-3; Roof plate-specific spondin-1

products gene name :

RSPO1

other gene names :

Rspo1; Rspo1; Rspondin; R-spondin; Cristin-3; mCristin-3

uniprot entry name :

RSPO1_MOUSE

reactivity :

Mouse

sequence length :

265

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse RSPO1. No significant cross-reactivity or interference between mouse RSPO1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 6.25 pg/ml -400 pg/ml. Sensitivity: The minimum detectable dose of mouse RSPO1 is typically less than 1.56 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for RSPO1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RSPO1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RSPO1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RSPO1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

227452351

ncbi acc num :

NP_619624.2

ncbi gb acc num :

NM_138683.2

uniprot acc num :

Q9Z132

ncbi mol weight :

29,332 Da

ncbi pathways :

Disease Pathway (970606); RNF Mutants Show Enhanced WNT Signaling And Proliferation Pathway (1000750); Signal Transduction Pathway (970540); Signaling By WNT In Cancer Pathway (1000719); Signaling By Wnt Pathway (1000988); TCF Dependent Signaling In Response To WNT Pathway (1000740); XAV939 Inhibits Tankyrase, Stabilizing AXIN Pathway (1000751); Misspliced LRP5 Mutants Have Enhanced Beta-catenin-dependent Signaling Pathway (1000739); Regulation Of FZD By Ubiquitination Pathway (1000747)

uniprot summary :

R-spondin-1: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination. Defects in RSPO1 are the cause of palmoplantar keratoderma with squamous cell carcinoma of skin and sex reversal (PKKSCC). This recessive syndrome is characterized by XX (female to male) SRY-independent sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin. Belongs to the R-spondin family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide; Cell adhesion; Cell development/differentiation. Cellular Component: extracellular space; extracellular region; nucleus. Molecular Function: heparin binding; protein binding; G-protein-coupled receptor binding; receptor binding. Biological Process: Wnt receptor signaling pathway; regulation of gene expression; regulation of receptor internalization; response to stimulus; Wnt receptor signaling pathway through beta-catenin; positive regulation of protein amino acid phosphorylation; male meiosis; positive regulation of Wnt receptor signaling pathway

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage