Human Protein FAN, NSMAF ELISA Kit | MyBioSource MBS9335966 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Protein FAN, NSMAF ELISA Kit

catalog :

MBS9335966

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS9335966

products type :

ELISA Kit

products full name :

Human Protein FAN, NSMAF ELISA Kit

products short name :

neutral sphingomyelinase (N-SMase) activation associated factor

products name syn :

Human Protein FAN (NSMAF) ELISA kit; FAN; ; neutral sphingomyelinase (N-SMase) activation associated factor

other names :

protein FAN isoform 2; Protein FAN; protein FAN; factor associated with N-SMase activation; factor associated with neutral sphingomyelinase activation; neutral sphingomyelinase (N-SMase) activation associated factor; Factor associated with neutral sphingomyelinase activation; Factor associated with N-SMase activation

products gene name :

NSMAF

other gene names :

NSMAF; NSMAF; FAN; FAN; Factor associated with N-SMase activation

uniprot entry name :

FAN_HUMAN

reactivity :

Human

specificity :

No significant cross-reactivity or interference between Human NSMAF and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 0.25 ng/ml - 8 ng/ml. Sensitivity: 0.1 ng/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated NSMAF concentrations in Human serum, plasma and other body fluids. Using Purified Human NSMAF antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add NSMAF and NSMAF antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NSMAF in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated NSMAF concentrations in Human serum, plasma and other body fluids. Using Purified Human NSMAF antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add NSMAF and NSMAF antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of NSMAF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

221625547

ncbi acc num :

NP_001138244.1

ncbi gb acc num :

NM_001144772.1

uniprot acc num :

Q92636

ncbi mol weight :

104,372 Da

ncbi pathways :

TNF Receptor Signaling Pathway (138053); TNF-alpha/NF-kB Signaling Pathway (198884)

ncbi summary :

This gene encodes a WD-repeat protein that binds the cytoplasmic sphingomyelinase activation domain of the 55kD tumor necrosis factor receptor. This protein is required for TNF-mediated activation of neutral sphingomyelinase and may play a role in regulating TNF-induced cellular responses such as inflammation. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2009]

uniprot summary :

NSMAF: Couples the p55 TNF-receptor (TNF-R55 / TNFR1) to neutral sphingomyelinase (N-SMASE). Specifically binds to the N- smase activation domain of TNF-R55. May regulate ceramide production by N-SMASE. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Adaptor/scaffold; Autophagy. Chromosomal Location of Human Ortholog: 8q12-q13. Cellular Component: extrinsic to membrane; cytoplasm; endomembrane system. Molecular Function: sphingomyelin phosphodiesterase activator activity; protein binding; receptor signaling protein activity; phospholipid binding. Biological Process: positive regulation of catalytic activity; positive regulation of apoptosis; ceramide metabolic process; endosome transport; signal transduction

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage