Human Chordin, CHRD ELISA Kit | MyBioSource MBS933551 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Chordin, CHRD ELISA Kit

catalog :

MBS933551

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS933551

products type :

ELISA Kit

products full name :

Human Chordin, CHRD ELISA Kit

products short name :

chordin

products name syn :

Human Chordin (CHRD) ELISA kit; MGC133038; ; chordin

other names :

chordin; Chordin; chordin; chordin

products gene name :

CHRD

other gene names :

CHRD; CHRD; UNQ217/PRO243

uniprot entry name :

CHRD_HUMAN

reactivity :

Human

sequence length :

955

specificity :

This assay has high sensitivity and excellent specificity for detection of human CHRD. No significant cross-reactivity or interference between human CHRD and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml -20 ng/ml. Sensitivity: The minimum detectable dose of human CHRD is typically less than 0.078 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CHRD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CHRD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CHRD is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CHRD bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

30089980

ncbi acc num :

NP_003732.2

ncbi gb acc num :

NM_003741.2

uniprot acc num :

Q9H2X0

ncbi mol weight :

102,032 Da

ncbi pathways :

BMP Receptor Signaling Pathway (137948); TGF-beta Signaling Pathway (83064); TGF-beta Signaling Pathway (475)

ncbi summary :

This gene encodes a secreted protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta-like bone morphogenetic proteins and sequestering them in latent complexes. The encoded protein may also have roles in organogenesis and during adulthood. Multiple transcript variants encoding distinct isoforms have been identified for this gene. Other alternative splice variants have been described but their full length sequence has not been determined. [provided by RefSeq, Jul 2008]

uniprot summary :

CHRD: Dorsalizing factor. Key developmental protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta family bone morphogenetic proteins (BMPs) and sequestering them in latent complexes. Belongs to the chordin family. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Cell adhesion; Secreted; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 3q27. Cellular Component: extracellular space. Molecular Function: syndecan binding; heparin binding; protein binding; cytokine binding. Biological Process: osteoblast differentiation; BMP signaling pathway in spinal cord dorsal-ventral patterning; floor plate development; positive regulation of cell adhesion; mesoderm formation; forebrain development; positive regulation of mesenchymal cell proliferation; negative regulation of osteoblast differentiation; gastrulation with mouth forming second; skeletal development; negative regulation of cell migration; negative regulation of BMP signaling pathway

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage