ELISA/assay

Human Bcl-2-like protein 11, BCL2L11 ELISA Kit

MBS933511

48-Strip-Wells

510 USD

ELISA Kit

Human Bcl-2-like protein 11, BCL2L11 ELISA Kit

BCL2-like 11 (apoptosis facilitator)

Human Bcl-2-like protein 11 (BCL2L11) ELISA kit; BAM; BIM; BIM-alpha6; BIM-beta6; BIM-beta7; BOD; BimEL; BimL; BCL2-like 11; OTTHUMP00000197013; bcl-2 interacting mediator of cell death; bcl-2 interacting protein Bim; bcl-2-related ovarian death agoni; BCL2-like 11 (apoptosis facilitator)

bcl-2-like protein 11 isoform 11; Bcl-2-like protein 11; bcl-2-like protein 11; bcl-2 interacting protein Bim; bcl-2-related ovarian death agonist; bcl-2 interacting mediator of cell death; BCL2-like 11 (apoptosis facilitator); Bcl2-interacting mediator of cell death

BCL2L11

BCL2L11; BCL2L11; BAM; BIM; BOD; BIM; Bcl2-L-11

B2L11_HUMAN

Human

198

This assay has high sensitivity and excellent specificity for detection of Human BCL2L11. No significant cross-reactivity or interference between Human BCL2L11 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 23.44 pg/ml-1500 pg/ml. Sensitivity: 5.86 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Apoptosis/Autophagy. Protein Biological Process 3: Apoptosis

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BCL2L11 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BCL2L11 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BCL2L11 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BCL2L11 bound in the initial step. The color development is stopped and the intensity of the color is measured.

323362959

NP_001191035.1

NM_001204106.1

O43521

22,171 Da

Activation Of BH3-only Proteins Pathway (105658); Activation Of BIM And Translocation To Mitochondria Pathway (105662); Apoptosis Pathway (198797); Apoptosis Pathway (105648); Apoptosis Modulation And Signaling Pathway (198822); B Cell Receptor Signaling Pathway (198909); BDNF Signaling Pathway (712093); BH3-only Proteins Associate With And Inactivate Anti-apoptotic BCL-2 Members Pathway (105664); Cell Death Signalling Via NRAGE, NRIF And NADE Pathway (106443); DNA Damage Response (only ATM Dependent) Pathway (198827)

The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The protein encoded by this gene contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the BCL-2 protein family and to act as an apoptotic activator. The expression of this gene can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role of this gene in neuronal and lymphocyte apoptosis. Transgenic studies of the mouse counterpart suggested that this gene functions as an essential initiator of apoptosis in thymocyte-negative selection. Several alternatively spliced transcript variants of this gene have been identified. [provided by RefSeq, Jun 2013]

BIM: a pro-apoptotic member of the BCL-2 protein family. Interacts with other members of the BCL-2 protein family, including BCL2, BCL2L1/BCL-X(L), and MCL1, and act as an apoptotic activator. Its expression can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role in neuronal and lymphocyte apoptosis. Transgenic studies in the mouse suggested that this protein functions as an essential initiator of the apoptosis in thymocyte-negative selection. Nineteen alternatively spliced transcript variants of this gene have been reported. Protein type: Apoptosis. Chromosomal Location of Human Ortholog: 2q13. Cellular Component: microtubule; mitochondrial outer membrane; extrinsic to membrane; endomembrane system; cytosol. Molecular Function: protein binding; microtubule binding. Biological Process: nerve growth factor receptor signaling pathway; positive regulation of apoptosis; apoptosis; cell-matrix adhesion; myeloid cell homeostasis; pigmentation during development; B cell apoptosis; positive regulation of caspase activity; ear development; cellular process regulating host cell cycle in response to virus; positive regulation of apoptosis by virus; B cell homeostasis; mammary gland development; positive regulation of neuron apoptosis; T cell homeostasis; kidney development; post-embryonic organ morphogenesis; caspase activation; spleen development; thymus development; in utero embryonic development; positive regulation of protein homooligomerization; male gonad development; positive regulation of cell cycle; lumen formation; regulation of organ growth; odontogenesis of dentine-containing teeth; DNA damage response, signal transduction resulting in induction of apoptosis; regulation of pigmentation during development; spermatogenesis; brain development

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800