Human Regulator of G-protein signaling 3, RGS3 ELISA Kit | MyBioSource MBS9334667 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Regulator of G-protein signaling 3, RGS3 ELISA Kit

catalog :

MBS9334667

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS9334667

products type :

ELISA Kit

products full name :

Human Regulator of G-protein signaling 3, RGS3 ELISA Kit

products short name :

regulator of G-protein signaling 3

products name syn :

Human Regulator of G-protein signaling 3 (RGS3) ELISA kit; C2PA; FLJ20370; FLJ31516; FLJ90496; PDZ-RGS3; RGP3; regulator of G-protein signalling 3; regulator of G-protein signaling 3

other names :

regulator of G-protein signaling 3 isoform 2; Regulator of G-protein signaling 3; regulator of G-protein signaling 3; regulator of G-protein signalling 3; regulator of G-protein signaling 3

products gene name :

RGS3

other gene names :

RGS3; RGS3; C2PA; RGP3; RGP3; RGS3

uniprot entry name :

RGS3_HUMAN

reactivity :

Human

specificity :

No significant cross-reactivity or interference between Human RGS3 and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 31.2 pg/ml - 1000 pg/ml. Sensitivity: 5.0 pg/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated RGS3 concentrations in Human serum, plasma and other body fluids. Using Purified Human RGS3 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add RGS3 and RGS3 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of RGS3 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated RGS3 concentrations in Human serum, plasma and other body fluids. Using Purified Human RGS3 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add RGS3 and RGS3 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of RGS3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

442654297

ncbi acc num :

NP_001263189.1

ncbi gb acc num :

NM_001276260.1

uniprot acc num :

P49796

ncbi mol weight :

132,336 Da

ncbi pathways :

Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Calcium Regulation In The Cardiac Cell Pathway (198906); Ephrin B Reverse Signaling Pathway (138052); Myometrial Relaxation And Contraction Pathways (198759)

ncbi summary :

This gene encodes a member of the regulator of G-protein signaling (RGS) family. This protein is a GTPase-activating protein that inhibits G-protein-mediated signal transduction. Alternative splicing and the use of alternative promoters results in multiple transcript variants encoding different isoforms. Long isoforms are largely cytosolic and plasma membrane-associated with a function in Wnt signaling and in the epithelial mesenchymal transition, while shorter N-terminally-truncated isoforms can be nuclear. [provided by RefSeq, Jan 2013]

uniprot summary :

RGS3: Down-regulates signaling from heterotrimeric G-proteins by increasing the GTPase activity of the alpha subunits, thereby driving them into their inactive GDP-bound form. Down-regulates G- protein-mediated release of inositol phosphates and activation of MAP kinases. 6 isoforms of the human protein are produced by alternative splicing. Protein type: GAPs, RGS; GAPs. Chromosomal Location of Human Ortholog: 9q32. Cellular Component: nucleoplasm; cytoplasm; plasma membrane; cytosol. Molecular Function: GTPase activator activity. Biological Process: regulation of G-protein coupled receptor protein signaling pathway; positive regulation of GTPase activity; inactivation of MAPK activity

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage