catalog number :
MBS9334148
products type :
ELISA Kit
products full name :
Human Testican-1, SPOCK1 ELISA Kit
products short name :
sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 1
products name syn :
Human Testican-1 (SPOCK1) ELISA kit; FLJ37170; SPOCK; TESTICAN; TIC1; OTTHUMP00000159433; sparc/osteonectin; cwcv and kazal-like domains proteoglycan 1; testican-1; sparc/osteonectin; cwcv and kazal-like domains proteoglycan (testican) 1
other names :
testican-1; Testican-1; testican-1; sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 1; Protein SPOCK
products gene name :
SPOCK1
other gene names :
SPOCK1; SPOCK1; TIC1; SPOCK; TESTICAN; SPOCK; TIC1; TICN1
uniprot entry name :
TICN1_HUMAN
specificity :
No significant cross-reactivity or interference between Human SPOCK1 and analogues was observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 31.2pg/ml-1000pg/ml. Sensitivity: 5.0pg/ml.
other info2 :
Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated SPOCK1 concentrations in Human serum, plasma and other body fluids. Using Purified Human SPOCK1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SPOCK1 and SPOCK1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SPOCK1 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
products description :
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated SPOCK1 concentrations in Human serum, plasma and other body fluids. Using Purified Human SPOCK1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SPOCK1 and SPOCK1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SPOCK1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
NP_004589.1
ncbi gb acc num :
NM_004598.3
ncbi mol weight :
49,124 Da
ncbi pathways :
Adipogenesis Pathway (198832)
ncbi summary :
This gene encodes the protein core of a seminal plasma proteoglycan containing chondroitin- and heparan-sulfate chains. The protein's function is unknown, although similarity to thyropin-type cysteine protease-inhibitors suggests its function may be related to protease inhibition. [provided by RefSeq, Jul 2008]
uniprot summary :
SPOCK1: May play a role in cell-cell and cell-matrix interactions. May contribute to various neuronal mechanisms in the central nervous system. Protein type: Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis; Cell adhesion. Chromosomal Location of Human Ortholog: 5q31.2. Cellular Component: proteinaceous extracellular matrix; extracellular space; cytoplasm; postsynaptic density; sarcoplasm; neuromuscular junction. Molecular Function: metalloendopeptidase inhibitor activity; serine-type endopeptidase inhibitor activity; calcium ion binding; cysteine protease inhibitor activity. Biological Process: nervous system development; central nervous system neuron differentiation; neurogenesis; neuron migration; regulation of cell growth; signal transduction; cell adhesion