Bovine A disintegrin and metalloproteinase with thrombospondin motifs 5, ADAMTS5 ELISA Kit | MyBioSource MBS933409 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Bovine A disintegrin and metalloproteinase with thrombospondin motifs 5, ADAMTS5 ELISA Kit

catalog :

MBS933409

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS933409

products type :

ELISA Kit

products full name :

Bovine A disintegrin and metalloproteinase with thrombospondin motifs 5, ADAMTS5 ELISA Kit

products short name :

ADAM metallopeptidase with thrombospondin type 1 motif, 5

products name syn :

Bovine A disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5) ELISA kit; ADAMTS11; ADMP-2; FLJ36738; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif; 5 (aggrecanase-2) ; aggrecanase-2; ADAM metallopeptidase with thrombospondin type 1 motif; 5

other names :

A disintegrin and metalloproteinase with thrombospondin motifs 5; A disintegrin and metalloproteinase with thrombospondin motifs 5; A disintegrin and metalloproteinase with thrombospondin motifs 5; ADMP-2; ADAM-TS5; ADAMTS-5; ADAM-TS 5; aggrecanase-2; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 5; ADAM metallopeptidase with thrombospondin type 1 motif, 5; ADMP-2; Aggrecanase-2

products gene name :

ADAMTS5

other gene names :

ADAMTS5; ADAMTS5; ADAM-TS 5; ADAM-TS5; ADAMTS-5

uniprot entry name :

ATS5_BOVIN

reactivity :

Bovine

sequence length :

207

specificity :

This assay has high sensitivity and excellent specificity for detection of bovine ADAMTS5. No significant cross-reactivity or interference between bovine ADAMTS5 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates and Cell lysates. Assay Type: Sandwich. Detection Range: 0.6 25 ng/ml -40 ng/ml. Sensitivity: The minimum detectable dose of bovine ADAMTS5 is typically less than 0.156 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADAMTS5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADAMTS5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAMTS5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAMTS5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

11131363

ncbi acc num :

Q9TT92.1

uniprot acc num :

Q9TT92

ncbi mol weight :

22,576 Da

ncbi pathways :

Degradation Of The Extracellular Matrix Pathway (1008851); Endochondral Ossification Pathway (920938); Extracellular Matrix Organization Pathway (1008838); Metabolism Of Proteins Pathway (942868); O-glycosylation Of TSR Domain-containing Proteins Pathway (1009558); O-linked Glycosylation Pathway (1009557); Post-translational Protein Modification Pathway (1009527)

uniprot summary :

Function: Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. May play a role in proteolytic processing mostly during the peri-implantation period. Catalytic activity: Cleaves aggrecan at the 392-Glu- -Ala-393 site. Cofactor: Binds 1 zinc ion per subunit

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage