catalog number :
MBS9333993
products type :
ELISA Kit
products full name :
Mouse Receptor-type tyrosine-protein phosphatase beta, PTPRB ELISA Kit
products short name :
protein tyrosine phosphatase, receptor type, B
products name syn :
Mouse Receptor-type tyrosine-protein phosphatase beta (PTPRB) ELISA kit; DKFZp686E2262; DKFZp686H15164; FLJ44133; HPTP-BETA; HPTPB; MGC142023; MGC59935; PTPB; R-PTP-BETA; VEPTP; protein tyrosine phosphatase; receptor type; beta polypeptide; protein tyrosine phosphatase; receptor type; B
other names :
receptor-type tyrosine-protein phosphatase beta; Receptor-type tyrosine-protein phosphatase beta; receptor-type tyrosine-protein phosphatase beta; R-PTP-beta; protein-tyrosine phosphatase beta; vascular endothelial protein tyrosine phosphatase; protein tyrosine phosphatase, receptor type, B; Vascular endothelial protein tyrosine phosphatase; VE-PTP
products gene name :
PTPRB
other gene names :
Ptprb; Ptprb; Ptpz; Rptpb; Veptp; VE-PTP; C130094E24; 3230402H02Rik; Protein-tyrosine phosphatase beta; R-PTP-beta; VE-PTP
uniprot entry name :
PTPRB_MOUSE
specificity :
No significant cross-reactivity or interference between Mouse PTPRB and analogues was observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 0.25 ng/ml - 8 ng/ml. Sensitivity: 0.1 ng/ml.
other info2 :
Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated PTPRB concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse PTPRB antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PTPRB and PTPRB antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PTPRB in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
products description :
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated PTPRB concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse PTPRB antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PTPRB and PTPRB antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PTPRB in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
NP_084204.2
ncbi gb acc num :
NM_029928.2
ncbi mol weight :
224,495 Da
ncbi pathways :
Adherens Junction Pathway (83267); Adherens Junction Pathway (481)
uniprot summary :
PTPRB: Plays an important role in blood vessel remodeling and angiogenesis. Not necessary for the initial formation of blood vessels, but is essential for their maintenance and remodeling. Can induce dephosphorylation of TEK/TIE2, CDH5/VE-cadherin and KDR/VEGFR-2. Regulates angiopoietin-TIE2 signaling in endothelial cells. Acts as a negative regulator of TIE2, and controls TIE2 driven endothelial cell proliferation, which in turn affects blood vessel remodeling during embryonic development and determines blood vessel size during perinatal growth. Essential for the maintenance of endothelial cell contact integrity and for the adhesive function of VE-cadherin in endothelial cells and this requires the presence of plakoglobin. Belongs to the protein-tyrosine phosphatase family. Receptor class 3 subfamily. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Receptor protein phosphatase, tyrosine; EC 3.1.3.48. Cellular Component: membrane; integral to membrane; receptor complex. Molecular Function: protein binding; hydrolase activity; phosphoric monoester hydrolase activity; protein tyrosine phosphatase activity; phosphoprotein phosphatase activity. Biological Process: dephosphorylation; angiogenesis; protein amino acid dephosphorylation