catalog number :
MBS933375
products type :
ELISA Kit
products full name :
Human Microfibrillar-associated protein 5, MFAP5 ELISA Kit
products short name :
microfibrillar associated protein 5
products name syn :
Human Microfibrillar-associated protein 5 (MFAP5) ELISA kit; MAGP2; MP25; microfibril-associated glycoprotein 2; microfibril-associated glycoprotein-2; microfibrillar associated protein 5
other names :
microfibrillar-associated protein 5 isoform a; Microfibrillar-associated protein 5; microfibrillar-associated protein 5; microfibril-associated glycoprotein 2; microfibril-associated glycoprotein-2; microfibrillar associated protein 5; MP25; Microfibril-associated glycoprotein 2; MAGP-2
products gene name :
MFAP5
other gene names :
MFAP5; MFAP5; MP25; MAGP2; MAGP-2; MFAP-5; MAGP2; MFAP-5; MAGP-2
uniprot entry name :
MFAP5_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human MFAP5. No significant cross-reactivity or interference between human MFAP5 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: 0.039 ng/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MFAP5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MFAP5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MFAP5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MFAP5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_003471.1
ncbi gb acc num :
NM_003480.3
ncbi mol weight :
19,612 Da
ncbi pathways :
Elastic Fibre Formation Pathway (730310); Extracellular Matrix Organization Pathway (576262); Molecules Associated With Elastic Fibres Pathway (730311); Notch Signaling Pathway (169345)
ncbi summary :
This gene encodes a 25-kD microfibril-associated glycoprotein which is a component of microfibrils of the extracellular matrix. The encoded protein promotes attachment of cells to microfibrils via alpha-V-beta-3 integrin. Deficiency of this gene in mice results in neutropenia. Alternate splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014]
uniprot summary :
MFAP5: Component of the elastin-associated microfibrils. Belongs to the MFAP family. Protein type: Secreted, signal peptide; Secreted; Extracellular matrix. Chromosomal Location of Human Ortholog: 12p13.1-p12.3. Cellular Component: extracellular region; microfibril. Molecular Function: extracellular matrix structural constituent. Biological Process: extracellular matrix organization and biogenesis; fibril organization and biogenesis. Disease: Aortic Aneurysm, Familial Thoracic 9
size4 :
10x96-Strip-Wells