catalog number :
MBS9333468
products type :
ELISA Kit
products full name :
Human LIM domain-containing protein 1, LIMD1 ELISA Kit
products short name :
[LIM domains containing 1]
products name syn :
[Human LIM domain-containing protein 1 (LIMD1) ELISA kit; LIM domains containing 1]
other names :
[LIM domain-containing protein 1; LIM domain-containing protein 1; LIM domain-containing protein 1; LIM domains containing 1]
products gene name :
[LIMD1]
other gene names :
[LIMD1; LIMD1]
uniprot entry name :
LIMD1_HUMAN
specificity :
No significant cross-reactivity or interference between this analyte and analogues is observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Body fluids, tissue homogenates, secretions or feces samples. Assay Type: Quantitative Sandwich. Detection Range: 6.25 ng/ml - 200 ng/ml. Sensitivity: 1.0 ng/ml.
other info2 :
Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
products description :
Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of LIMD1 (hereafter termed "analyte") in undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.
ncbi acc num :
NP_055055.1
ncbi gb acc num :
NM_014240.2
ncbi mol weight :
72,190 Da
ncbi pathways :
Hippo Signaling Pathway (749777); Hippo Signaling Pathway (750388)
uniprot summary :
LIMD1: Adapter or scaffold protein which participates in the assembly of numerous protein complexes and is involved in several cellular processes such as cell fate determination, cytoskeletal organization, repression of gene transcription, cell-cell adhesion, cell differentiation, proliferation and migration. Positively regulates microRNA (miRNA)-mediated gene silencing and is essential for P-body formation and integrity. Acts as a hypoxic regulator by bridging an association between the prolyl hydroxylases and VHL enabling efficient degradation of HIF1A. Acts as a transcriptional corepressor for SNAI1- and SNAI2/SLUG- dependent repression of E-cadherin transcription. Negatively regulates the Hippo signaling pathway and antagonizes phosphorylation of YAP1. Inhibits E2F-mediated transcription, and suppresses the expression of the majority of genes with E2F1- responsive elements. Regulates osteoblast development, function, differentiation and stress osteoclastogenesis. Enhances the ability of TRAF6 to activate adapter protein complex 1 (AP-1) and negatively regulates the canonical Wnt receptor signaling pathway in osteoblasts. May act as a tumor suppressor by inhibiting cell proliferation. Belongs to the zyxin/ajuba family. Protein type: Tumor suppressor; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 3p21.3. Cellular Component: focal adhesion; adherens junction; cytoplasm; nucleus. Molecular Function: protein binding; zinc ion binding; transcription corepressor activity. Biological Process: cell migration; transcription, DNA-dependent; multicellular organismal development; cytoskeleton organization and biogenesis; miRNA-mediated gene silencing; signal transduction; osteoblast development; regulation of cell shape; regulation of transcription, DNA-dependent; response to hypoxia; negative regulation of osteoblast differentiation; cytoplasmic mRNA processing body assembly; negative regulation of transcription, DNA-dependent; phosphorylation
size4 :
10x96-Strip-Wells