ELISA/assay

Mouse EGF-like module-containing mucin-like hormone receptor-like 1, EMR1 ELISA Kit

MBS933313

48-Strip-Wells

510 USD

ELISA Kit

Mouse EGF-like module-containing mucin-like hormone receptor-like 1, EMR1 ELISA Kit

egf-like module containing, mucin-like, hormone receptor-like 1

Mouse EGF-like module-containing mucin-like hormone receptor-like 1 (EMR1) ELISA kit; TM7LN3; egf-like module containing; mucin-like; hormone receptor-like sequence 1; egf-like module containing; mucin-like; hormone receptor-like 1

EGF-like module-containing mucin-like hormone receptor-like 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGF-like module-containing mucin-like hormone receptor-like 1; EMR1 hormone receptor; lymphocyte antigen 71; EGF-like module receptor 1; cell surface glycoprotein F4/80; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; Cell surface glycoprotein F4/80; EGF-like module receptor 1; EMR1 hormone receptor

EMR1

Emr1; Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7; Gpf480

EMR1_MOUSE

Mouse

931

This assay has high sensitivity and excellent specificity for detection of mouse EMR1. No significant cross-reactivity or interference between mouse EMR1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 7.81 pg/ml-500 pg/ml. Sensitivity: The minimum detectable dose of mouse EMR1 is typically less than 1.95 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EMR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EMR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EMR1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EMR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

33859546

NP_034260.1

NM_010130.4

Q61549

102,130 Da

GPCRs, Class B Secretin-like Pathway (198364); Non-odorant GPCRs Pathway (198395)

EMR1: Could be involved in cell-cell interactions. Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 2; Membrane protein, integral. Cellular Component: membrane; plasma membrane; integral to membrane; external side of plasma membrane. Molecular Function: G-protein coupled receptor activity; signal transducer activity; transmembrane receptor activity; calcium ion binding. Biological Process: G-protein coupled receptor protein signaling pathway; cell surface receptor linked signal transduction; signal transduction

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800