catalog number :
MBS933254
products type :
ELISA Kit
products full name :
Human HERV-K_7p22.1 provirus ancestral Env polyprotein, ERVK6 ELISA Kit
products short name :
endogenous retroviral sequence K, 6
products name syn :
Human HERV-K_7p22.1 provirus ancestral Env polyprotein (ERVK6) ELISA kit; HERV-K108; ; endogenous retroviral sequence K; 6
other names :
HERV-K_7p22.1 provirus ancestral Env polyprotein; HERV-K_7p22.1 provirus ancestral Env polyprotein; EnvK2 protein; Envelope polyprotein; HERV-K(C7) envelope protein; HERV-K(HML-2.HOM) envelope protein; HERV-K108 envelope proteinCleaved into the following 2 chains:Surface protein; SU; Transmembrane protein; TM
products gene name :
ERVK6
other gene names :
ERVK6; SU; TM
uniprot entry name :
ENK2_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human ERVK6. No significant cross-reactivity or interference between human ERVK6 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: 0.039 ng/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ERVK6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ERVK6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ERVK6 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ERVK6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi mol weight :
79,218 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
uniprot summary :
ERVK6: Retroviral envelope proteins mediate receptor recognition and membrane fusion during early infection. Endogenous envelope proteins may have kept, lost or modified their original function during evolution. This endogenous envelope protein has lost its original fusogenic properties. Belongs to the beta type-B retroviral envelope protein family. HERV class-II K(HML-2) env subfamily. Protein type: EC 3.4.23.16; EC 3.4.23.-; EC 2.7.7.7; Protease; EC 3.1.26.4; Transferase; Membrane protein, integral; EC 2.7.7.49; Ribonuclease. Chromosomal Location of Human Ortholog: 7p22.1
size4 :
10x96-Strip-Wells