This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
MyBioSource
product type :
ELISA/assay
product name :
Bovine Calpain-1 Catalytic subunit, CAPN1 ELISA Kit
catalog :
MBS933204
quantity :
48-Strip-Wells
price :
565 USD
product information
catalog number :
MBS933204
products type :
ELISA Kit
products full name :
Bovine Calpain-1 Catalytic subunit, CAPN1 ELISA Kit
products short name :
calpain 1, (mu/I) large subunit
products name syn :
Bovine Calpain-1 catalytic subunit (CAPN1) ELISA kit; CANP; CANP1; CANPL1; muCANP; muCL; calcium-activated neutral proteinase; calpain 1; large subunit; calpain; large polypeptide L1; cell proliferation-inducing protein 30; calpain 1; (mu/I) large subunit
other names :
calpain-1 catalytic subunit; Calpain-1 catalytic subunit; calpain-1 catalytic subunit; CANP 1; muCANP; mu-calpain; calpain mu-type; micromolar-calpain; calpain-1 large subunit; calcium-activated neutral proteinase 1; micromolar calcium activated neutral protease 1; micromolar calcium-activated neutral protease 1; calpain 1, (mu/I) large subunit; Calcium-activated neutral proteinase 1; CANP 1; Calpain mu-type; Calpain-1 large subunit; Micromolar-calpain; muCANP
products gene name :
CAPN1
other gene names :
CAPN1; CAPN1; CANP 1; muCANP
uniprot entry name :
CAN1_BOVIN
reactivity :
Bovine
sequence length :
716
specificity :
This assay has high sensitivity and excellent specificity for detection of bovine CAPN1. No significant cross-reactivity or interference between bovine CAPN1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 34.4 pg/ml-2200 pg/ml. Sensitivity: The minimum detectable dose of bovine CAPN1 is typically less than 8.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CAPN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CAPN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CAPN1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CAPN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
27806281
ncbi acc num :
NP_776684.1
ncbi gb acc num :
NM_174259.2
uniprot acc num :
Q27970
ncbi mol weight :
82,207 Da
ncbi pathways :
Alzheimer's Disease Pathway (84336); Alzheimer's Disease Pathway (509); Apoptosis Pathway (84299); Apoptosis Pathway (470); Degradation Of The Extracellular Matrix Pathway (1008851); Extracellular Matrix Organization Pathway (1008838); Integrin-mediated Cell Adhesion Pathway (198555); Protein Processing In Endoplasmic Reticulum Pathway (167316); Protein Processing In Endoplasmic Reticulum Pathway (167190)
uniprot summary :
Function: Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Catalytic activity: Broad endopeptidase specificity. Cofactor: Binds 4 calcium ions . Enzyme regulation: Activated by micromolar concentrations of calcium and inhibited by calpastatin.
size1 :
48-Strip-Wells
price1 :
565 USD
size2 :
96-Strip-Wells
price2 :
810
size3 :
5x96-Strip-Wells
price3 :
2750
size4 :
10x96-Strip-Wells
price4 :
5150
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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