catalog number :
MBS9331927
products type :
ELISA Kit
products full name :
Human Ribonuclease H1, RNASEH1 ELISA Kit
products short name :
[ribonuclease H1]
products name syn :
[Human Ribonuclease H1 (RNASEH1) ELISA kit; H1RNA; RNH1; RNase H1; ribonuclease H type II; ribonuclease H1]
other names :
[ribonuclease H1 isoform 2; Ribonuclease H1; ribonuclease H1; RNase H1; ribonuclease H type II; ribonuclease H1; Ribonuclease H type II]
products gene name :
[RNASEH1]
other gene names :
[RNASEH1; RNASEH1; RNH1; H1RNA; RNH1; RNase H1]
uniprot entry name :
RNH1_HUMAN
specificity :
No significant cross-reactivity or interference between this analyte and analogues is observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Body fluids, tissue homogenates, secretions or feces samples. Assay Type: Quantitative Sandwich. Detection Range: 0.625 ng/ml - 20 ng/ml. Sensitivity: 0.1 ng/ml.
other info2 :
Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
products description :
Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of RNASEH1 (hereafter termed "analyte") in undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.
ncbi acc num :
NP_001273763.1
ncbi gb acc num :
NM_001286834.1
ncbi mol weight :
32,064 Da
ncbi pathways :
DNA Replication Pathway (83039); DNA Replication Pathway (444)
ncbi summary :
This gene encodes an endonuclease that specifically degrades the RNA of RNA-DNA hybrids and is necessary for DNA replication and repair. This enzyme is present in both mitochondria and nuclei, which are resulted from translation of a single mRNA with two in-frame initiation start codons. The use of the first start codon produces the mitochondrial isoform and the use of the second start codon produces the nuclear isoform. The production of the mitochondrial isoform is modulated by an upstream open reading frame (uORF) which overlaps the first initiation start codon in human. An alternately spliced transcript variant has been found which encodes a shorter isoform. This gene has three pseudogenes; two of them are on different locations of chromosome 17 and one of them is on chromosome 1q32.2. [provided by RefSeq, Nov 2013]
uniprot summary :
RNASEH1: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids. Belongs to the RNase H family. Protein type: RNA-binding; Ribonuclease; EC 3.1.26.4; Mitochondrial. Chromosomal Location of Human Ortholog: 2p25. Cellular Component: mitochondrion; nucleus. Molecular Function: protein homodimerization activity; nucleic acid binding; RNA binding; magnesium ion binding; ribonuclease H activity; ribonuclease activity. Biological Process: RNA catabolic process; mitochondrial DNA replication; termination of RNA polymerase II transcription. Disease: Progressive External Ophthalmoplegia With Mitochondrial Dna Deletions, Autosomal Recessive 2
size4 :
10x96-Strip-Wells