catalog number :
MBS9331736
products type :
ELISA Kit
products full name :
Mouse Neutral ceramidase, ASAH2 ELISA Kit
products short name :
[N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2]
products name syn :
[Mouse Neutral ceramidase (ASAH2) ELISA kit; BCDase; HNAC1; MGC129777; NCDase; N-acylsphingosine amidohydrolase 2; mitochondrial ceramidase; neutral ceramidase; neutral/alkaline ceramidase; non-lysosomal ceramidase; N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2]
other names :
[neutral ceramidase; Neutral ceramidase; neutral ceramidase; NCDase; N-CDase; acylsphingosine deacylase 2; neutral/alkaline ceramidase; N-acylsphingosine amidohydrolase 2; Acylsphingosine deacylase 2; N-acylsphingosine amidohydrolase 2Cleaved into the following chain:Neutral ceramidase soluble form]
products gene name :
[ASAH2]
other gene names :
[Asah2; Asah2; AI585898; N-CDase; NCDase]
uniprot entry name :
ASAH2_MOUSE
specificity :
No significant cross-reactivity or interference between this analyte and analogues is observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Undiluted original Mouse body fluids, tissue homogenates, secretions or feces samples. Assay Type: Sandwich (Quantitative). Detection Range: 0.625 ng/ml - 20 ng/ml. Sensitivity: 0.1 ng/ml.
other info2 :
Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
products description :
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of ASAH2 (hereafter termed "analyte") in undiluted original Mouse body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.
ncbi acc num :
NP_061300.1
ncbi gb acc num :
NM_018830.1
ncbi mol weight :
83,509 Da
ncbi pathways :
Signal Transduction Of S1P Receptor Pathway (198402); Sphingolipid Metabolism Pathway (83193); Sphingolipid Metabolism Pathway (369); Sphingosine Biosynthesis Pathway (524686); Sphingosine Biosynthesis Pathway (468292); Sphingosine And Sphingosine-1-phosphate Metabolism Pathway (542862); Sphingosine And Sphingosine-1-phosphate Metabolism Pathway (138547)
uniprot summary :
ASAH2: Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract. Belongs to the neutral ceramidase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.5.1.23; Lipid Metabolism - sphingolipid; Membrane protein, integral; Hydrolase. Cellular Component: mitochondrion; membrane; plasma membrane; integral to membrane. Molecular Function: hydrolase activity; ceramidase activity. Biological Process: response to organic substance; cell proliferation; sphingolipid metabolic process; apoptosis; lipid metabolic process
size4 :
10x96-Strip-Wells