Human Rap1 GTPase-activating protein 1, RAP1GAP ELISA Kit | MyBioSource MBS9330527 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Rap1 GTPase-activating protein 1, RAP1GAP ELISA Kit

catalog :

MBS9330527

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS9330527

products type :

ELISA Kit

products full name :

Human Rap1 GTPase-activating protein 1, RAP1GAP ELISA Kit

products short name :

RAP1 GTPase activating protein

products name syn :

Human Rap1 GTPase-activating protein 1 (RAP1GAP) ELISA kit; RP11-63N8.1; KIAA0474; RAP1GA1; RAP1GAP1; RAP1GAPII; RAPGAP; RAP1; GTPase activating protein 1; RAP1 GTPase activating protein

other names :

rap1 GTPase-activating protein 1 isoform b; Rap1 GTPase-activating protein 1; rap1 GTPase-activating protein 1; RAP1 GTPase activating protein

products gene name :

RAP1GAP

other gene names :

RAP1GAP; RAP1GAP; RAPGAP; RAP1GA1; RAP1GAP1; RAP1GAPII; KIAA0474; RAP1GA1; Rap1GAP; Rap1GAP1

uniprot entry name :

RPGP1_HUMAN

reactivity :

Human

specificity :

No significant cross-reactivity or interference between Human RAP1GAP1 and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 31.2 pg/ml - 1000 pg/ml. Sensitivity: 5.0 pg/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated RAP1GAP1 concentrations in Human serum, plasma and other body fluids. Using Purified Human RAP1GAP1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add RAP1GAP1 and RAP1GAP1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of RAP1GAP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated RAP1GAP1 concentrations in Human serum, plasma and other body fluids. Using Purified Human RAP1GAP1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add RAP1GAP1 and RAP1GAP1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of RAP1GAP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

224809573

ncbi acc num :

NP_001139129.1

ncbi gb acc num :

NM_001145657.1

uniprot acc num :

P47736

ncbi mol weight :

73,361 Da

ncbi pathways :

Adaptive Immune System Pathway (366160); IL-5 Signaling Pathway (198770); Immune System Pathway (106386); Rap1 Signalling Pathway (187108)

ncbi summary :

This gene encodes a type of GTPase-activating-protein (GAP) that down-regulates the activity of the ras-related RAP1 protein. RAP1 acts as a molecular switch by cycling between an inactive GDP-bound form and an active GTP-bound form. The product of this gene, RAP1GAP, promotes the hydrolysis of bound GTP and hence returns RAP1 to the inactive state whereas other proteins, guanine nucleotide exchange factors (GEFs), act as RAP1 activators by facilitating the conversion of RAP1 from the GDP- to the GTP-bound form. In general, ras subfamily proteins, such as RAP1, play key roles in receptor-linked signaling pathways that control cell growth and differentiation. RAP1 plays a role in diverse processes such as cell proliferation, adhesion, differentiation, and embryogenesis. Alternative splicing results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Aug 2011]

uniprot summary :

RAP1GAP: a GTPase activator for the nuclear Ras-related regulatory protein RAP-1A (KREV-1), converting it to the putatively inactive GDP-bound state. Protein type: Motility/polarity/chemotaxis; GAPs, Ras; GAPs. Chromosomal Location of Human Ortholog: 1p36.1-p35. Cellular Component: Golgi membrane; membrane; cytosol. Molecular Function: GTPase activity; protein binding; protein homodimerization activity; Ras GTPase binding; GTPase activator activity. Biological Process: metabolic process; signal transduction

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage