catalog number :
MBS9330249
products type :
ELISA Kit
products full name :
Human Kelch-like protein 22, KLHL22 ELISA Kit
products short name :
[kelch-like 22 (Drosophila)]
products name syn :
[Human Kelch-like protein 22 (KLHL22) ELISA kit; KELCHL; kelch-like; kelch-like 22 (Drosophila)]
other names :
[kelch-like protein 22; Kelch-like protein 22; kelch-like protein 22; kelch-like 22; kelch-like family member 22]
products gene name :
[KLHL22]
other gene names :
[KLHL22; KLHL22; KELCHL]
uniprot entry name :
KLH22_HUMAN
specificity :
No significant cross-reactivity or interference between Human KLHL22 and analogues was observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 6.25 ng/ml - 200 ng/ml. Sensitivity: 1.0 ng/ml.
other info2 :
Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
products description :
Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated KLHL22 concentrations in Human serum, plasma and other body fluids. Using Purified Human KLHL22 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add KLHL22 and KLHL22 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of KLHL22 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
NP_116164.2
ncbi gb acc num :
NM_032775.3
ncbi mol weight :
71,667 Da
uniprot summary :
KLHL22: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for cell division. BCR E3 ubiquitin ligase complexes mediate the ubiquitination of target proteins. Chromosomal Location of Human Ortholog: 22q11.21. Cellular Component: centrosome; cytoplasm; polar microtubule. Molecular Function: protein binding. Biological Process: protein monoubiquitination; mitotic sister chromatid segregation; cell division; mitotic cell cycle spindle assembly checkpoint
size4 :
10x96-Strip-Wells