ELISA/assay

Human GTP-binding protein Rheb, RHEB ELISA Kit

MBS9330069

48-Strip-Wells

470 USD

ELISA Kit

Human GTP-binding protein Rheb, RHEB ELISA Kit

[Ras homolog enriched in brain]

[Human GTP-binding protein Rheb (RHEB) ELISA kit; MGC111559; RHEB2; GTP-binding protein Rheb; Ras homolog enriched in brain 2; Ras homolog enriched in brain]

[GTP-binding protein Rheb; GTP-binding protein Rheb; GTP-binding protein Rheb; Ras homolog enriched in brain 2; Ras homolog enriched in brain; Ras homolog enriched in brain]

[RHEB]

[RHEB; RHEB; RHEB2; RHEB2]

RHEB_HUMAN

Human

No significant cross-reactivity or interference between this analyte and analogues is observed.

Store all reagents at 2-8 degree C

Samples: Undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances. Assay Type: Sandwich. Detection Range: 3.12ng/ml-100ng/ml. Sensitivity: 1.0ng/ml.

Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, NOT for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of RHEB (hereafter termed "analyte") in undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.

5032041

NP_005605.1

NM_005614.3

Q15382

20,497 Da

Energy Dependent Regulation Of MTOR By LKB1-AMPK Pathway (160972); IRS-mediated Signalling Pathway (106426); IRS-related Events Pathway (106424); Insulin Signaling Pathway (198845); Insulin Receptor Signalling Cascade Pathway (106420); Insulin Signaling Pathway (83090); Insulin Signaling Pathway (501); PI3K Cascade Pathway (106427); PKB-mediated Events Pathway (106428); Regulation Of Rheb GTPase Activity By AMPK Pathway (160974)

This gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22. [provided by RefSeq, Jul 2008]

RHEB: Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Activates the protein kinase activity of mTORC1. Has low intrinsic GTPase activity. Binds to mTORC1 in a guanyl nucleotide-independent manner. Interacts directly with MTOR, MLST8 and RPTOR. Interacts with TSC2. Ubiquitous. Highest levels observed in skeletal and cardiac muscle. Alternates between an inactive form bound to GDP and an active form bound to GTP. Inactivated by TSC1-TSC2 via the GTPase activating protein (GAP) domain of TSC2. Belongs to the small GTPase superfamily. Rheb family. Protein type: G protein; G protein, monomeric, Rheb; G protein, monomeric. Chromosomal Location of Human Ortholog: 7q36. Cellular Component: Golgi membrane; spliceosome; endoplasmic reticulum membrane; cell soma; membrane; dendrite; plasma membrane; cytosol. Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding; metal ion binding; protein kinase binding. Biological Process: metabolic process; small GTPase mediated signal transduction; insulin receptor signaling pathway; cell cycle arrest; signal transduction; positive regulation of TOR signaling pathway

48-Strip-Wells

470 USD

96-Strip-Wells

680

5x96-Strip-Wells

3100

10x96-Strip-Wells

6095