ELISA/assay

Human Mitotic checkpoint serine/threonine-protein kinase BUB1 beta, BUB1B ELISA Kit

MBS9329895

48-Strip-Wells

470 USD

ELISA Kit

Human Mitotic checkpoint serine/threonine-protein kinase BUB1 beta, BUB1B ELISA Kit

[budding uninhibited by benzimidazoles 1 homolog beta (yeast)]

[Human Mitotic checkpoint serine/threonine-protein kinase BUB1 beta (BUB1B) ELISA kit; BUB1beta; BUBR1; Bub1A; MAD3L; SSK1; hBUBR1; BUB1 budding uninhibited by benzimidazoles 1 homolog beta; MAD3/BUB1-related protein kinase; budding uninhibited by benzimidazoles 1 beta; budding uninhibit; budding uninhibited by benzimidazoles 1 homolog beta (yeast)]

[mitotic checkpoint serine/threonine-protein kinase BUB1 beta; Mitotic checkpoint serine/threonine-protein kinase BUB1 beta; mitotic checkpoint serine/threonine-protein kinase BUB1 beta; mitotic checkpoint kinase MAD3L; MAD3/BUB1-related protein kinase; budding uninhibited by benzimidazoles 1 homolog beta; BUB1 mitotic checkpoint serine/threonine kinase B; MAD3/BUB1-related protein kinase; hBUBR1; Mitotic checkpoint kinase MAD3L; Protein SSK1]

[BUB1B]

[BUB1B; BUB1B; MVA1; SSK1; BUBR1; Bub1A; MAD3L; hBUBR1; BUB1beta; BUBR1; MAD3L; SSK1; hBUBR1]

BUB1B_HUMAN

Human

No significant cross-reactivity or interference between this analyte and analogues is observed.

Store all reagents at 2-8 degree C

Samples: Undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances. Assay Type: Sandwich. Detection Range: 0.625 ng/ml - 20 ng/ml. Sensitivity: 0.1 ng/ml.

Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, NOT for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of BUB1B (hereafter termed "analyte") in undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.

59814247

NP_001202.4

NM_001211.5

O60566

119,545 Da

APC/C-mediated Degradation Of Cell Cycle Proteins Pathway (105825); APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway (105831); Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway (105829); Cell Cycle Pathway (530733); Cell Cycle Checkpoints Pathway (105739); Cell Cycle, Mitotic Pathway (105765); Cell Cycle Pathway (198811); Cell Cycle Pathway (83054); Cell Cycle Pathway (463); DNA Replication Pathway (105897)

This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer. [provided by RefSeq, Jul 2008]

BUB1B: Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression. Interacts with CENPE, CENPF, mitosin, PLK1 and BUB3. Part of a complex containing BUB3, CDC20 and BUB1B. Interacts with anaphase-promoting complex/cyclosome (APC/C). Interacts with CASC5. Induced during mitosis. Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index. Kinase activity stimulated by CENPE. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. BUB1 subfamily. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Tumor suppressor; EC 2.7.11.1; Kinase, protein; Other group; BUB family. Chromosomal Location of Human Ortholog: 15q15. Cellular Component: kinetochore; anaphase-promoting complex; perinuclear region of cytoplasm; cytoplasm; microtubule organizing center; outer kinetochore of condensed chromosome; spindle midzone; cytosol. Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity. Biological Process: cell proliferation; mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; apoptosis; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; mitotic cell cycle checkpoint; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle; protein amino acid phosphorylation; mitotic metaphase/anaphase transition. Disease: Mosaic Variegated Aneuploidy Syndrome 1; Premature Chromatid Separation Trait

48-Strip-Wells

470 USD

96-Strip-Wells

680

5x96-Strip-Wells

3100

10x96-Strip-Wells

6095