ELISA/assay

Monkey Macrophage Inflammatory Protein-3alpha, MIP-3alpha/CCL20 ELISA Kit

MBS932985

48-Strip-Wells

565 USD

ELISA Kit

Monkey Macrophage Inflammatory Protein-3alpha, MIP-3alpha/CCL20 ELISA Kit

Macrophage Inflammatory Protein-3alpha (MIP-3alpha/CCL20)

Monkey Macrophage Inflammatory Protein-3alpha (MIP-3alpha/CCL20) ELISA Kit; CKb4; LARC; MIP-3a; MIP3A; SCYA20; ST38; exodus-1; macrophage inflammatory protein 3 alpha; small inducible cytokine subfamily A (Cys-Cys); member 20; Macrophage Inflammatory Protein-3alpha (MIP-3alpha/CCL20)

C-C motif chemokine 20 isoform 2; C-C motif chemokine 20; C-C motif chemokine 20; CC chemokine LARC; beta chemokine exodus-1; small-inducible cytokine A20; macrophage inflammatory protein 3 alpha; liver and activation-regulated chemokine; small inducible cytokine subfamily A (Cys-Cys), member 20; chemokine (C-C motif) ligand 20; Beta-chemokine exodus-1; CC chemokine LARC; Liver and activation-regulated chemokine; Macrophage inflammatory protein 3 alpha; MIP-3-alpha; Small-inducible cytokine A20Cleaved into the following 3 chains:CCL20(1-67); CCL20(1-64); CCL20(2-70)

MIP-3alpha/CCL20

CCL20; CCL20; CKb4; LARC; ST38; MIP3A; MIP-3a; SCYA20; MIP-3-alpha; LARC; MIP3A; SCYA20; MIP-3-alpha

CCL20_HUMAN

Monkey

95

This assay has high sensitivity and excellent specificity for detection of monkey MIP-3alpha/CCL20. No significant cross-reactivity or interference between monkey MIP-3alpha/CCL20 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml -2000 pg/ml. Sensitivity: The minimum detectable dose of monkey MIP-3alpha/CCL20 is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

. Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MIP-3alpha/CCL20 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MIP-3alpha/CCL20 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MIP-3alpha/CCL20 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MIP-3alpha/CCL20 bound in the initial step. The color development is stopped and the intensity of the color is measured.

194239688

NP_001123518.1

NM_001130046.1

P78556

10,762 Da

Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); EBV LMP1 Signaling Pathway (198790); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020)

This antimicrobial gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The protein encoded by this gene displays chemotactic activity for lymphocytes and can repress proliferation of myeloid progenitors. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]

CCL20: Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213. Belongs to the intercrine beta (chemokine CC) family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted; Chemokine; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 2q36.3. Cellular Component: extracellular space; extracellular region. Molecular Function: protein binding; chemokine activity. Biological Process: cell-cell signaling; wound healing; defense response to bacterium; immune response; positive regulation of interleukin-1 alpha biosynthetic process; signal transduction; inflammatory response; chemotaxis; positive regulation of nitric-oxide synthase biosynthetic process

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150