catalog number :
MBS932900
products type :
ELISA Kit
products full name :
Human Kielin/chordin-like protein, KCP ELISA Kit
products short name :
kielin/chordin-like protein
products name syn :
Human Kielin/chordin-like protein (KCP) ELISA kit; CRIM2; KCP1; NET67; cysteine rich BMP regulator 2; cysteine rich BMP regulator 2 (chordin-like) ; kielin; kielin-like; kielin/chordin-like protein 1; kielin/chordin-like protein
other names :
kielin/chordin-like protein isoform 1; Kielin/chordin-like protein; kielin/chordin-like protein; KCP-1; CRIM-2; cysteine-rich BMP regulator 2; kielin/chordin-like protein 1; cysteine-rich motor neuron 2 protein; cysteine rich BMP regulator 2 (chordin-like); kielin/chordin-like protein; Cysteine-rich BMP regulator 2; Cysteine-rich motor neuron 2 protein; CRIM-2; Kielin/chordin-like protein 1; KCP-1
other gene names :
KCP; KCP; KCP1; CRIM2; NET67; CRIM2; KCP1; CRIM-2; KCP-1
uniprot entry name :
KCP_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human KCP. No significant cross-reactivity or interference between human KCP and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.18 ng/ml -12 ng/ml. Sensitivity: The minimum detectable dose of human KCP is typically less than 0.04 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for KCP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KCP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KCP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KCP bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001129386.1
ncbi gb acc num :
NM_001135914.1
ncbi mol weight :
159,938 Da
uniprot summary :
CRIM2: Enhances bone morphogenetic protein (BMP) signaling in a paracrine manner. In contrast, it inhibits both the activin-A and TGFB1-mediated signaling pathways. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 7q32.1. Cellular Component: extracellular region
size4 :
10x96-Strip-Wells