Human Myocardin, MYOCD ELISA Kit | MyBioSource MBS9328433 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Myocardin, MYOCD ELISA Kit

catalog :

MBS9328433

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS9328433

products type :

ELISA Kit

products full name :

Human Myocardin, MYOCD ELISA Kit

products short name :

myocardin

products name syn :

Human Myocardin (MYOCD) ELISA kit; MYCD; ; myocardin

other names :

myocardin isoform 1; Myocardin; myocardin; myocardin

products gene name :

MYOCD

other gene names :

MYOCD; MYOCD; MYCD; MYCD

uniprot entry name :

MYCD_HUMAN

reactivity :

Human

specificity :

No significant cross-reactivity or interference between Human MYOCD and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 0.25 ng/ml - 8 ng/ml. Sensitivity: 0.1 ng/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated MYOCD concentrations in Human serum, plasma and other body fluids. Using Purified Human MYOCD antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MYOCD and MYOCD antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MYOCD in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated MYOCD concentrations in Human serum, plasma and other body fluids. Using Purified Human MYOCD antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MYOCD and MYOCD antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MYOCD in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

226423889

ncbi acc num :

NP_001139784.1

ncbi gb acc num :

NM_001146312.2

uniprot acc num :

Q8IZQ8

ncbi mol weight :

101,997 Da

ncbi summary :

This gene encodes a nuclear protein, which is expressed in heart, aorta, and in smooth muscle cell-containing tissues. It functions as a transcriptional co-activator of serum response factor (SRF) and modulates expression of cardiac and smooth muscle-specific SRF-target genes, and thus may play a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]

uniprot summary :

myocardin: Smooth muscle cells (SM) and cardiac muscle cells- specific transcriptional factor which uses the canonical single or multiple CArG boxes DNA sequence. Acts as a cofactor of serum response factor (SRF) with the potential to modulate SRF-target genes. Plays a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage (myogenesis). 3 isoforms of the human protein are produced by alternative splicing. Protein type: Cell development/differentiation; Transcription factor. Chromosomal Location of Human Ortholog: 17p11.2. Cellular Component: nucleus. Molecular Function: histone acetyltransferase binding; protein binding; histone deacetylase binding; transcription coactivator activity; transcription factor binding. Biological Process: transcription from RNA polymerase II promoter; regulation of cell growth by extracellular stimulus; positive regulation of transcription, DNA-dependent; ventricular cardiac muscle cell differentiation; negative regulation of transcription from RNA polymerase II promoter; uterus development; cardiac muscle cell differentiation; negative regulation of cell proliferation; regulation of smooth muscle cell differentiation; positive regulation of cell proliferation; positive regulation of smooth muscle contraction; vasculogenesis; cardiac cell differentiation; positive regulation of DNA binding; urinary bladder development; positive regulation of smooth muscle cell differentiation; positive regulation of transforming growth factor beta receptor signaling pathway; negative regulation of cyclin-dependent protein kinase activity; regulation of histone acetylation; gut development; regulation of myoblast differentiation; response to hypoxia; positive regulation of transcription from RNA polymerase II promoter; smooth muscle cell differentiation; positive regulation of transcription factor activity; alveolus development

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage