Rat Gamma-aminobutyric acid receptor-associated protein-like 1, GABARAPL1 ELISA Kit | MyBioSource MBS9328399 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Gamma-aminobutyric acid receptor-associated protein-like 1, GABARAPL1 ELISA Kit

catalog :

MBS9328399

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS9328399

products type :

ELISA Kit

products full name :

Rat Gamma-aminobutyric acid receptor-associated protein-like 1, GABARAPL1 ELISA Kit

products short name :

GABA (A) receptor-associated protein like 1

products name syn :

Rat Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1) ELISA kit; APG8-LIKE; APG8L; ATG8; ATG8L; GEC1; early estrogen-regulated protein; GABA (A) receptor-associated protein like 1

other names :

gamma-aminobutyric acid receptor-associated protein-like 1; Gamma-aminobutyric acid receptor-associated protein-like 1; gamma-aminobutyric acid receptor-associated protein-like 1; GEC-1; glandular epithelial cell protein 1; GABA(A) receptor-associated protein-like 1; gamma-aminobutyric acid (GABA(A)) receptor-associated protein-like 1; GABA(A) receptor-associated protein like 1; GABA(A) receptor-associated protein-like 1; Glandular epithelial cell protein 1; GEC-1

products gene name :

GABARAPL1

other gene names :

Gabarapl1; Gabarapl1; Gec1; Gec1; GEC-1

uniprot entry name :

GBRL1_RAT

reactivity :

Rat

specificity :

No significant cross-reactivity or interference between Rat GABARAPL1 and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 0.5 ng/ml - 16 ng/ml. Sensitivity: 0.1 ng/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated GABARAPL1 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat GABARAPL1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GABARAPL1 and GABARAPL1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GABARAPL1 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated GABARAPL1 concentrations in Rat serum, plasma and other body fluids. Using Purified Rat GABARAPL1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GABARAPL1 and GABARAPL1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GABARAPL1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

112982886

ncbi acc num :

NP_001037759.1

ncbi gb acc num :

NM_001044294.1

uniprot acc num :

Q0VGK0

ncbi mol weight :

14,044 Da

ncbi pathways :

GABAergic Synapse Pathway (377244); GABAergic Synapse Pathway (377129); Regulation Of Autophagy Pathway (83450); Regulation Of Autophagy Pathway (468)

uniprot summary :

GABARAPL1: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. Increases cell-surface expression of kappa-type opioid receptor through facilitating anterograde intracellular trafficking of the receptor. Belongs to the MAP1 LC3 family. Interacts with GABRG2 and beta-tubulin. Interacts with KOR-1. Protein type: Ubiquitin-like modifier; Vesicle; Autophagy; Microtubule-binding; Adaptor/scaffold

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage