Human Structural maintenance of chromosomes protein 1B, SMC1B ELISA Kit | MyBioSource MBS9328004 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Structural maintenance of chromosomes protein 1B, SMC1B ELISA Kit

catalog :

MBS9328004

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS9328004

products type :

ELISA Kit

products full name :

Human Structural maintenance of chromosomes protein 1B, SMC1B ELISA Kit

products short name :

structural maintenance of chromosomes 1B

products name syn :

Human Structural maintenance of chromosomes protein 1B (SMC1B) ELISA kit; RP1-102D24.3; FLJ43748; SMC1BETA; SMC1L2; bK268H5; bK268H5.5; SMC1 (structural maintenance of chromosomes 1; yeast) -like 1; SMC1 structural maintenance of chromosomes 1-like 2; mitosis-specific chromo; structural maintenance of chromosomes 1B

other names :

structural maintenance of chromosomes protein 1B isoform 2; Structural maintenance of chromosomes protein 1B; structural maintenance of chromosomes protein 1B; SMC protein 1B; SMC1 structural maintenance of chromosomes 1-like 2; SMC1 (structural maintenance of chromosomes 1, yeast)-like 1; mitosis-specific chromosome segregation protein like protein beta; structural maintenance of chromosomes 1B

products gene name :

SMC1B

other gene names :

SMC1B; SMC1B; SMC1L2; SMC1BETA; SMC1L2; SMC protein 1B; SMC-1-beta; SMC-1B

uniprot entry name :

SMC1B_HUMAN

reactivity :

Human

specificity :

No significant cross-reactivity or interference between Human SMC1B and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 0.625 ng/ml - 20 ng/ml. Sensitivity: 0.1 ng/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated SMC1B concentrations in Human serum, plasma and other body fluids. Using Purified Human SMC1B antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SMC1B and SMC1B antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SMC1B in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated SMC1B concentrations in Human serum, plasma and other body fluids. Using Purified Human SMC1B antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SMC1B and SMC1B antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SMC1B in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

613410201

ncbi acc num :

NP_001278430.1

ncbi gb acc num :

NM_001291501.1

uniprot acc num :

Q8NDV3

ncbi mol weight :

143,908 Da

ncbi pathways :

Cell Cycle Pathway (530733); Cell Cycle Pathway (83054); Cell Cycle Pathway (463); Meiosis Pathway (477133); Meiotic Synapsis Pathway (477134); Oocyte Meiosis Pathway (126909); Oocyte Meiosis Pathway (126872)

ncbi summary :

SMC1L2 belongs to a family of proteins required for chromatid cohesion and DNA recombination during meiosis and mitosis (3:Revenkova et al., 2001 [PubMed 11564881]).[supplied by OMIM, Mar 2008]

uniprot summary :

SMC1B: Meiosis-specific component of cohesin complex. Required for the maintenance of meiotic cohesion, but not, or only to a minor extent, for its establishment. Contributes to axial element (AE) formation and the organization of chromatin loops along the AE. Plays a key role in synapsis, recombination and chromosome movements. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The meiosis-specific cohesin complex probably replaces mitosis specific cohesin complex when it dissociates from chromatin during prophase I. Belongs to the SMC family. SMC1 subfamily. 3 isoforms of the human protein are produced by alternative splicing. Chromosomal Location of Human Ortholog: 22q13.31. Cellular Component: nucleoplasm; lateral element; cytoplasm; chromosome, pericentric region. Molecular Function: DNA binding; ATP binding. Biological Process: meiosis; sister chromatid cohesion

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage