Human Low affinity immunoglobulin gamma Fc region receptor II-b, FCGR2B ELISA Kit | MyBioSource MBS932794 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Low affinity immunoglobulin gamma Fc region receptor II-b, FCGR2B ELISA Kit

catalog :

MBS932794

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS932794

products type :

ELISA Kit

products full name :

Human Low affinity immunoglobulin gamma Fc region receptor II-b, FCGR2B ELISA Kit

products short name :

Fc fragment of IgG, low affinity IIb, receptor (CD32)

products name syn :

Human Low affinity immunoglobulin gamma Fc region receptor II-b (FCGR2B) ELISA kit; CD32; CD32B; FCG2; FCGR2; IGFR2; Fc fragment of IgG; low affinity II; receptor for (CD32) ; Fc fragment of IgG; low affinity IIb; receptor; Fc fragment of IgG; low affinity IIb; receptor for (CD32) ; Fc; Fc fragment of IgG; low affinity IIb; receptor (CD32)

other names :

low affinity immunoglobulin gamma Fc region receptor II-b isoform 2; Low affinity immunoglobulin gamma Fc region receptor II-b; low affinity immunoglobulin gamma Fc region receptor II-b; CDw32; fcRII-b; Fc gamma RIIb; fc-gamma-RIIb; fc-gamma RII-b; igG Fc receptor II-b; Fc fragment of IgG, low affinity II, receptor for (CD32); Fc fragment of IgG, low affinity IIb, receptor for (CD32); Fc fragment of IgG, low affinity IIb, receptor (CD32); CDw32; Fc-gamma RII-b; Fc-gamma-RIIb; FcRII-b; CD_antigen: CD32

products gene name :

FCGR2B

other gene names :

FCGR2B; FCGR2B; CD32; FCG2; CD32B; FCGR2; IGFR2; CD32; FCG2; IGFR2; IgG Fc receptor II-b; Fc-gamma-RIIb; FcRII-b

uniprot entry name :

FCG2B_HUMAN

reactivity :

Human

sequence length :

290

specificity :

This assay has high sensitivity and excellent specificity for detection of human FCGR2B. No significant cross-reactivity or interference between human FCGR2B and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates and cell lysates. Detection Range: 0.625 ng/ml -40 ng/ml. Sensitivity: The minimum detectable dose of human FCGR2B is typically less than 0.156 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Assay Type: Sandwich

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FCGR2B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FCGR2B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FCGR2B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FCGR2B bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

50511932

ncbi acc num :

NP_001002273.1

ncbi gb acc num :

NM_001002273.2

uniprot acc num :

P31994

ncbi mol weight :

34,044 Da

ncbi pathways :

Adaptive Immune System Pathway (366160); B Cell Receptor Signaling Pathway (198909); B Cell Receptor Signaling Pathway (83081); B Cell Receptor Signaling Pathway (492); BCR Signaling Pathway (138058); Fc Gamma R-mediated Phagocytosis Pathway (114228); Fc Gamma R-mediated Phagocytosis Pathway (106590); Fc-epsilon Receptor I Signaling In Mast Cells Pathway (138050); Immune System Pathway (106386); Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway (106413)

ncbi summary :

The protein encoded by this gene is a low affinity receptor for the Fc region of immunoglobulin gamma complexes. The encoded protein is involved in the phagocytosis of immune complexes and in the regulation of antibody production by B-cells. Variations in this gene may increase susceptibilty to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]

uniprot summary :

FCGR2B: a transmembrane receptor for the Fc region of complexed or aggregated immunoglobulin gamma (IgG). Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down modulation of previous state of cell activation triggered via antigen receptors on B cells, T cells or via another Fc receptor. Three splice-variant isoforms have been observed. Protein type: Cell surface; Oncoprotein; Membrane protein, integral. Chromosomal Location of Human Ortholog: 1q23. Cellular Component: integral to membrane; plasma membrane. Molecular Function: protein binding; IgG binding. Biological Process: regulation of immune response; viral reproduction; immune response; signal transduction. Disease: Systemic Lupus Erythematosus; Malaria, Susceptibility To

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage