ELISA/assay

Mouse TRAF2 and NCK-interacting protein kinase, TNIK ELISA Kit

MBS9327669

48-Strip-Wells

470 USD

ELISA Kit

Mouse TRAF2 and NCK-interacting protein kinase, TNIK ELISA Kit

[TRAF2 and NCK interacting kinase]

[Mouse TRAF2 and NCK-interacting protein kinase (TNIK) ELISA kit; TRAF2 and NCK interacting kinase]

[traf2 and NCK-interacting protein kinase isoform 3; Traf2 and NCK-interacting protein kinase; traf2 and NCK-interacting protein kinase; TRAF2 and NCK interacting kinase]

[TNIK]

[Tnik; Tnik; AI451411; 1500031A17Rik; 4831440I19Rik; C530008O15Rik; C630040K21Rik; Kiaa0551]

TNIK_MOUSE

Mouse

No significant cross-reactivity or interference between this analyte and analogues is observed.

Store all reagents at 2-8 degree C

Samples: Body fluids, tissue homogenates, secretions or feces samples. Assay Type: Quantitative Sandwich. Detection Range: 3.12 ng/ml - 100 ng/ml. Sensitivity: 1.0 ng/ml.

Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of TNIK (hereafter termed "analyte") in undiluted original Mouse body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.

244792613

NP_001156480.1

NM_001163008.1

P83510

150,367 Da

TNIK: Serine/threonine kinase that acts as an essential activator of the Wnt signaling pathway. Recruited to promoters of Wnt target genes and required to activate their expression. May act by phosphorylating TCF4/TCF7L2. Appears to act upstream of the JUN N-terminal pathway. May play a role in the response to environmental stress. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it may play a role in cytoskeletal rearrangements and regulate cell spreading. Phosphorylates SMAD1 on Thr-322. Interacts (via the CNH domain) with RAP2A (GTP-bound form preferentially); the interaction is direct and required for the activation of TNIK by RAP2A. Interacts with NEDD4; recruits RAP2A to NEDD4. Interacts with TRAF2 and NCK. Interacts with TCF7L2/TCF4 and CTNNB1; the interaction is direct. Interacts with TANC1. Expressed ubiquitously. Highest levels observed in heart, brain and skeletal muscle. Expressed in normal colonic epithelia and colorectal cancer tissues. Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. 8 isoforms of the human protein are produced by alternative splicing. Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, STE; Kinase, protein; STE group; STE20 family; MSN subfamily. Cellular Component: nucleoplasm; recycling endosome; cytoskeleton; perinuclear region of cytoplasm; apical plasma membrane; cytoplasm; nucleus; endosome. Molecular Function: transferase activity; protein serine/threonine kinase activity; protein binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; ATP binding; protein kinase activity; receptor signaling protein serine/threonine kinase activity. Biological Process: nervous system development; microvillus biogenesis; Wnt receptor signaling pathway; protein amino acid autophosphorylation; regulation of dendrite morphogenesis; actin cytoskeleton reorganization; response to stress; cytoskeleton organization and biogenesis; positive regulation of protein amino acid phosphorylation; phosphorylation; protein amino acid phosphorylation; activation of JNKK activity

48-Strip-Wells

470 USD

96-Strip-Wells

680

5x96-Strip-Wells

3100

10x96-Strip-Wells

6095