ELISA/assay

Human V-type proton ATPase 116 kDa subunit a isoform 4, ATP6V0A4 ELISA Kit

MBS9327390

48-Strip-Wells

470 USD

ELISA Kit

Human V-type proton ATPase 116 kDa subunit a isoform 4, ATP6V0A4 ELISA Kit

[ATPase, H+ transporting, lysosomal V0 subunit a4]

[Human V-type proton ATPase 116 kDa subunit a isoform 4 (ATP6V0A4) ELISA kit; tcag7.346; A4; ATP6N1B; ATP6N2; MGC130016; MGC130017; RDRTA2; RTA1C; RTADR; STV1; VPH1; VPP2; ATPase; H+ transporting; lysosomal (vacuolar proton pump) non-catalytic accessory protein 1B; ATPase; H+; ATPase; H+ transporting; lysosomal V0 subunit a4]

[V-type proton ATPase 116 kDa subunit a isoform 4; V-type proton ATPase 116 kDa subunit a isoform 4; V-type proton ATPase 116 kDa subunit a isoform 4; V-ATPase 116 kDa; vacuolar proton pump, subunit 2; vacuolar proton pump 116 kDa accessory subunit; vacuolar proton translocating ATPase 116 kDa subunit a kidney isoform; H(+)-transporting two-sector ATPase, noncatalytic accessory protein 1B; ATPase, H+ transporting, lysosomal (vacuolar proton pump) non-catalytic accessory protein 1B; ATPase, H+ transporting, lysosomal (vacuolar proton pump) non-catalytic accessory protein 2 (38kD); ATPase, H+ transporting, lysosomal V0 subunit a4; Vacuolar proton translocating ATPase 116 kDa subunit a isoform 4; Vacuolar proton translocating ATPase 116 kDa subunit a kidney isoform]

[ATP6V0A4]

[ATP6V0A4; ATP6V0A4; A4; STV1; VPH1; VPP2; RTA1C; RTADR; ATP6N2; RDRTA2; ATP6N1B; ATP6N1B; ATP6N2; V-ATPase 116 kDa isoform a4]

VPP4_HUMAN

Human

No significant cross-reactivity or interference between this analyte and analogues is observed.

Store all reagents at 2-8 degree C

Samples: Body fluids, tissue homogenates, secretions or feces samples. Assay Type: Quantitative Sandwich. Detection Range: 0.625 ng/ml - 20 ng/ml. Sensitivity: 0.1 ng/ml.

Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of ATP6V0A4 (hereafter termed "analyte") in undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.

85386053

NP_065683.2

NM_020632.2

Q9HBG4

96,386 Da

Collecting Duct Acid Secretion Pathway (147586); Collecting Duct Acid Secretion Pathway (147560); Epithelial Cell Signaling In Helicobacter Pylori Infection Pathway (83102); Epithelial Cell Signaling In Helicobacter Pylori Infection Pathway (515); Insulin Receptor Recycling Pathway (106438); Iron Uptake And Transport Pathway (187191); Lysosome Pathway (99052); Lysosome Pathway (96865); Oxidative Phosphorylation Pathway (82942); Oxidative Phosphorylation Pathway (303)

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. This gene is one of four genes in man and mouse that encode different isoforms of the a subunit. Alternatively spliced transcript variants encoding the same protein have been described. Mutations in this gene are associated with renal tubular acidosis associated with preserved hearing. [provided by RefSeq, Jul 2008]

ATP6V0A4: Part of the proton channel of the V-ATPase that is involved in normal vectorial acid transport into the urine by the kidney. Defects in ATP6V0A4 are the cause of distal renal tubular acidosis with preserved hearing (RTADR). RTADR is an autosomal recessive form of distal renal tubular acidosis (dRTA), a group of disorders characterized by functional failure of alpha- intercalated cells of the cortical collecting duct of the distal nephron, where vectorial proton transport is required for urinary acidification. Functional failure of alpha-intercalated cells results in metabolic acidosis accompanied by disturbances of potassium balance, urinary calcium solubility, bone physiology and growth. Belongs to the V-ATPase 116 kDa subunit family. Protein type: Transporter, ion channel; Membrane protein, integral; Transporter; Energy Metabolism - oxidative phosphorylation; Membrane protein, multi-pass; Transporter, iron. Chromosomal Location of Human Ortholog: 7q34. Cellular Component: phagocytic vesicle membrane; brush border membrane; apical part of cell; lysosomal membrane; apical plasma membrane; integral to membrane; plasma membrane; endosome membrane; vacuolar proton-transporting V-type ATPase complex; endosome. Molecular Function: protein binding; hydrogen ion transporting ATPase activity, rotational mechanism; ATPase binding. Biological Process: vacuolar acidification; interaction with host; ATP synthesis coupled proton transport; ossification; proton transport; sensory perception of sound; cellular iron ion homeostasis; ATP hydrolysis coupled proton transport; insulin receptor signaling pathway; transferrin transport; regulation of pH; excretion; transmembrane transport. Disease: Renal Tubular Acidosis, Distal, Autosomal Recessive

48-Strip-Wells

470 USD

96-Strip-Wells

680

5x96-Strip-Wells

3100

10x96-Strip-Wells

6095