catalog number :
MBS9327285
products type :
ELISA Kit
products full name :
Rabbit Rho-associated protein kinase 1, ROCK1 ELISA Kit
products short name :
[Rho-associated, coiled-coil containing protein kinase 1]
products name syn :
[Rabbit Rho-associated protein kinase 1 (ROCK1) ELISA kit; MGC131603; MGC43611; P160ROCK; PRO0435; Rho kinase; p160-ROCK; renal carcinoma antigen NY-REN-35; Rho-associated; coiled-coil containing protein kinase 1]
other names :
[rho-associated protein kinase 1; Rho-associated protein kinase 1; rho-associated protein kinase 1; HEBM1; cePKA; p160ROCK; p160 ROCK-1; cAMP-dependent protein kinase ROCK-I; corneal epithelial Rho-associated-Ser/Thr kinase 1; rho-associated, coiled-coil-containing protein kinase 1; rho-associated, coiled-coil-containing protein kinase I; Corneal epithelial Rho-associated-Ser/Thr kinase 1; HEBM1; Rho-associated, coiled-coil-containing protein kinase 1; Rho-associated, coiled-coil-containing protein kinase I; ROCK-I; cAMP-dependent protein kinase ROCK-I; CePKA; p160 ROCK-1; p160ROCK]
products gene name :
[ROCK1]
other gene names :
[ROCK1; ROCK1; ROCK-I; ROCK-I; CePKA; p160ROCK]
uniprot entry name :
ROCK1_RABIT
specificity :
No significant cross-reactivity or interference between this analyte and analogues is observed.
storage stability :
Store all reagents at 2-8 degree C
other info1 :
Assay Type: Quantitative Sandwich. Detection Range: 3.12 ng/ml - 100 ng/ml. Sensitivity: 1.0 ng/ml
other info2 :
Intra-assay Precision: Intra-assay CV(%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
products description :
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of ROCK1 (hereafter termed "analyte") in undiluted original Rabbit body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.
ncbi acc num :
NP_001075836.1
ncbi gb acc num :
NM_001082367.1
ncbi mol weight :
158,347 Da
uniprot summary :
Function: Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization 1 PublicationManual assertion based on experiment in:Ref.2. Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Cofactor: Magnesium . Enzyme regulation: Activated by RHOA binding. Inhibited by Y-27632
size4 :
10x96-Strip-Wells