ELISA/assay

Human Alpha-ketoglutarate-dependent dioxygenase FTO, FTO ELISA Kit

MBS9326824

48-Strip-Wells

470 USD

ELISA Kit

Human Alpha-ketoglutarate-dependent dioxygenase FTO, FTO ELISA Kit

[fat mass and obesity associated]

[Human Alpha-ketoglutarate-dependent dioxygenase FTO (FTO) ELISA kit; KIAA1752; MGC5149; ; fat mass and obesity associated]

[alpha-ketoglutarate-dependent dioxygenase FTO; Alpha-ketoglutarate-dependent dioxygenase FTO; alpha-ketoglutarate-dependent dioxygenase FTO; protein fto; AlkB homolog 9; fat mass and obesity-associated protein; fat mass and obesity associated; Fat mass and obesity-associated protein]

[FTO]

[FTO; FTO; ALKBH9; KIAA1752]

FTO_HUMAN

Human

No significant cross-reactivity or interference between this analyte and analogues is observed.

Store all reagents at 2-8 degree C

Samples: Rat Body Fluids And Tissue Homogenates. Assay Type: Quantitative Sandwich. Detection Range: 0.25mmol/L-8mmol/L. Sensitivity: 0.1mmol/L.

Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.

Background/Introduction: This Quantitative Sandwich ELISA kit is for lab reagent/research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determine the level of FTA (hereafter termed "analyte") in undiluted original Rat body fluids and tissue homogenates. This kit is NOT suitable for assaying non-biological sources of substances.

122937263

NP_001073901.1

NM_001080432.2

Q9C0B1

12,218 Da

This gene is a nuclear protein of the AlkB related non-haem iron and 2-oxoglutarate-dependent oxygenase superfamily but the exact physiological function of this gene is not known. Other non-heme iron enzymes function to reverse alkylated DNA and RNA damage by oxidative demethylation. Studies in mice and humans indicate a role in nervous and cardiovascular systems and a strong association with body mass index, obesity risk, and type 2 diabetes. [provided by RefSeq, Jul 2011]

FTO: Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single- stranded RNA containing 3-methyluracil, followed by single- stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation. Defects in FTO are the cause of growth retardation developmental delay coarse facies and early death (GDFD). A severe polymalformation syndrome characterized by postnatal growth retardation, microcephaly, severe psychomotor delay, functional brain deficits and characteristic facial dysmorphism. In some patients, structural brain malformations, cardiac defects, genital anomalies, and cleft palate are observed. Early death occurs by the age of 3 years. Belongs to the fto family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: EC 1.14.11.-. Chromosomal Location of Human Ortholog: 16q12.2. Cellular Component: nuclear speck; nucleus. Molecular Function: ferrous iron binding; DNA-N1-methyladenine dioxygenase activity. Biological Process: DNA dealkylation; RNA repair; regulation of multicellular organism growth; thermoregulation. Disease: Growth Retardation, Developmental Delay, Coarse Facies, And Early Death

48-Strip-Wells

470 USD

96-Strip-Wells

680

5x96-Strip-Wells

3100

10x96-Strip-Wells

6095