Mouse DNA mismatch repair protein Msh3, MSH3 ELISA Kit | MyBioSource MBS9326679 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse DNA mismatch repair protein Msh3, MSH3 ELISA Kit

catalog :

MBS9326679

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS9326679

products type :

ELISA Kit

products full name :

Mouse DNA mismatch repair protein Msh3, MSH3 ELISA Kit

products short name :

mutS homolog 3 (E. coli)

products name syn :

Mouse DNA mismatch repair protein Msh3 (MSH3) ELISA kit; DUP; MGC163306; MGC163308; MRP1; Divergent upstream protein; Mismatch repair protein 1; mutS homolog 3; mutS homolog 3 (E. coli)

other names :

DNA mismatch repair protein Msh3; DNA mismatch repair protein Msh3; DNA mismatch repair protein Msh3; REP-1; protein repair-1; protein repair-3; mutS homolog 3 (E. coli); Protein repair-1; REP-1; Protein repair-3; REP-3

products gene name :

MSH3

other gene names :

Msh3; Msh3; Rep3; Rep-3; D13Em1; Rep-3; REP-1; REP-3

uniprot entry name :

MSH3_MOUSE

reactivity :

Mouse

specificity :

No significant cross-reactivity or interference between Mouse MSH3 and analogues was observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine. Assay Type: Sandwich. Detection Range: 1.2 pg/ml - 1000 pg/ml. Sensitivity: 5.0 pg/ml.

other info2 :

Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated MSH3 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse MSH3 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MSH3 and MSH3 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MSH3 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

products description :

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated MSH3 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse MSH3 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add MSH3 and MSH3 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MSH3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

68299763

ncbi acc num :

NP_034959.2

ncbi gb acc num :

NM_010829.2

uniprot acc num :

P13705

ncbi mol weight :

123,074 Da

ncbi pathways :

Colorectal Cancer Pathway (83299); Colorectal Cancer Pathway (518); Mismatch Repair Pathway (83242); Mismatch Repair Pathway (453); Pathways In Cancer (83298)

uniprot summary :

MSH3: Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion- deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. Defects in MSH3 are a cause of susceptibility to endometrial cancer (ENDMC). Belongs to the DNA mismatch repair MutS family. MSH3 subfamily. Protein type: DNA repair, damage. Cellular Component: nuclear chromosome; membrane; nucleus; MutSbeta complex. Molecular Function: DNA-dependent ATPase activity; double-strand/single-strand DNA junction binding; dinucleotide repeat insertion binding; nucleotide binding; loop DNA binding; mismatched DNA binding; Y-form DNA binding; centromeric DNA binding; enzyme binding; DNA binding; single guanine insertion binding; damaged DNA binding; dinucleotide insertion or deletion binding; single-stranded DNA binding; ATP binding. Biological Process: positive regulation of helicase activity; negative regulation of DNA recombination; mismatch repair; meiotic recombination; somatic hypermutation of immunoglobulin genes; somatic recombination of immunoglobulin gene segments; DNA repair; response to DNA damage stimulus; maintenance of DNA repeat elements; meiotic mismatch repair

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

600

more info or order :

MyBioSource product webpage