ELISA/assay

Mouse Serine/threonine-protein kinase ULK1, ULK1 ELISA Kit

MBS9326629

48-Strip-Wells

435 USD

ELISA Kit

Mouse Serine/threonine-protein kinase ULK1, ULK1 ELISA Kit

unc-51-like kinase 1 (C. elegans)

Mouse Serine/threonine-protein kinase ULK1 (ULK1) ELISA kit; ATG1; FLJ38455; FLJ46475; UNC51; Unc51.1; ATG1 autophagy related 1 homolog; Unc-51-like kinase 1; unc-51-like kinase 1 (C. elegans)

serine/threonine-protein kinase ULK1; Serine/threonine-protein kinase ULK1; serine/threonine-protein kinase ULK1; unc-51-like kinase 1; serine/threonine-protein kinase Unc51.1; unc-51 like kinase 1; Serine/threonine-protein kinase Unc51.1; Unc-51-like kinase 1

ULK1

Ulk1; Ulk1; Unc51.1; AU041434; mKIAA0722

ULK1_MOUSE

Mouse

No significant cross-reactivity or interference between this analyte and analogues is observed.

Store all reagents at 2-8 degree C

Samples: Undiluted original Mouse body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances. Assay Type: Sandwich. Detection Range: 0.5ng/ml-16ng/ml. Sensitivity: 0.1ng/ml.

Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, NOT for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of ULK1 (hereafter termed "analyte") in undiluted original Mouse body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.

40254402

NP_033495.2

NM_009469.3

O70405

112,463 Da

PI3K/Akt/mTOR Signaling Pathway (522982); Regulation Of Autophagy Pathway (83255); Regulation Of Autophagy Pathway (468); Senescence And Autophagy Pathway (198361); MTOR Signaling Pathway (83256); MTOR Signaling Pathway (469)

ULK1: Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3- kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. Interacts with GABARAP and GABARAPL2. Interacts (via C- terminus) with ATG13/KIAA0652. Part of a complex consisting of ATG13/KIAA0652, ULK1 and RB1CC1. Associates with the mammalian target of rapamycin complex 1 (mTORC1) through an interaction with RPTOR; the association depends on nutrient conditions and is reduced during starvation. Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily. Protein type: Autophagy; Kinase, protein; EC 2.7.11.1; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Other group; ULK family. Cellular Component: neuron projection; cytoplasmic vesicle membrane; cell soma; membrane; pre-autophagosomal structure membrane; cytoplasm; autophagic vacuole; cytosol. Molecular Function: transferase activity; protein serine/threonine kinase activity; protein binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; protein complex binding; kinase activity; Hsp90 protein binding; protein kinase binding; ATP binding; Rab GTPase binding; protein kinase activity. Biological Process: axon extension; protein amino acid autophosphorylation; neurite regeneration; negative regulation of collateral sprouting; signal transduction; cerebellar granule cell differentiation; protein amino acid phosphorylation; radial glia guided migration of granule cell; response to starvation; peptidyl-serine phosphorylation; cellular response to nutrient levels; protein localization; axonogenesis; regulation of gene expression; receptor internalization; Ras protein signal transduction; autophagy; regulation of autophagy; regulation of nerve growth factor receptor signaling pathway; positive regulation of macroautophagy; phosphorylation; neurite development; positive regulation of autophagy; autophagic vacuole formation

48-Strip-Wells

435 USD

96-Strip-Wells

600