ELISA/assay

Human Proteasome subunit beta type-5, PSMB5 ELISA Kit

MBS9326560

48-Strip-Wells

470 USD

ELISA Kit

Human Proteasome subunit beta type-5, PSMB5 ELISA Kit

[proteasome (prosome, macropain) subunit, beta type, 5]

[Human Proteasome subunit beta type-5 (PSMB5) ELISA kit; LMPX; MB1; MGC104214; X; PSX large multifunctional protease X; macropain epsilon chain; multicatalytic endopeptidase complex epsilon chain; proteasome beta 5 subunit; proteasome catalytic subunit 3; prot; proteasome (prosome; macropain) subunit; beta type; 5]

[proteasome subunit beta type-5 isoform 2; Proteasome subunit beta type-5; proteasome subunit beta type-5; proteasome chain 6; proteasome subunit X; proteasome subunit MB1; macropain epsilon chain; proteasome epsilon chain; proteasome beta 5 subunit; proteasome catalytic subunit 3; proteasome subunit, beta type, 5; PSX large multifunctional protease X; multicatalytic endopeptidase complex epsilon chain; proteasome (prosome, macropain) subunit, beta type, 5; Macropain epsilon chain; Multicatalytic endopeptidase complex epsilon chain; Proteasome chain 6; Proteasome epsilon chain; Proteasome subunit MB1; Proteasome subunit X]

[PSMB5]

[PSMB5; PSMB5; X; MB1; LMPX; LMPX; MB1; X]

PSB5_HUMAN

Human

No significant cross-reactivity or interference between this analyte and analogues is observed.

Store all reagents at 2-8 degree C

Samples: Human Serum, Plasma Or Tissue Homogenates Samples. Assay Type: Quantitative Sandwich. Detection Range: 0.5ng/ml-16ng/ml. Sensitivity: 0.1ng/ml.

Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.

Background/Introduction: This Quantitative Sandwich ELISA kit is for lab reagent/research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determine the level of PSMB4 (hereafter termed "analyte") in undiluted original Human serum, plasma or tissue homogenates samples. For other sample types please contact tech support to determine compatibility with this assay. This kit is not suitable for assaying non-biological sources of substances.

195539356

NP_001124197.1

NM_001130725.1

P28074

28,480 Da

APC/C-mediated Degradation Of Cell Cycle Proteins Pathway (105825); APC/C:Cdc20 Mediated Degradation Of Securin Pathway (105833); APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway (105831); APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway (105835); Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway (105829); Activation Of NF-kappaB In B Cells Pathway (576251); Adaptive Immune System Pathway (366160); Antigen Processing-Cross Presentation Pathway (477122); Antigen Processing: Ubiquitination Proteasome Degradation Pathway (366162); Apoptosis Pathway (105648)

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 3i (proteasome beta 8 subunit). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009]

PSMB5: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the chymotrypsin-like activity of the proteasome and is one of the principal target of the proteasome inhibitor bortezomib. May catalyze basal processing of intracellular antigens. Plays a role in the protection against oxidative damage through the Nrf2-ARE pathway. Belongs to the peptidase T1B family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.4.25.1; Proteasome complex; Protease. Chromosomal Location of Human Ortholog: 14q11.2. Cellular Component: nucleoplasm; proteasome complex; proteasome core complex; centrosome; cytosol; nucleus. Molecular Function: threonine endopeptidase activity; protein binding. Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; response to oxidative stress; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis

48-Strip-Wells

470 USD

96-Strip-Wells

680

5x96-Strip-Wells

3100

10x96-Strip-Wells

6095