Mouse Ornithine carbamoyltransferase, mitochondrial, OTC ELISA Kit | MyBioSource MBS932651 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Ornithine carbamoyltransferase, mitochondrial, OTC ELISA Kit

catalog :

MBS932651

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS932651

products type :

ELISA Kit

products full name :

Mouse Ornithine carbamoyltransferase, mitochondrial, OTC ELISA Kit

products short name :

ornithine carbamoyltransferase

products name syn :

Mouse Ornithine carbamoyltransferase; mitochondrial (OTC) ELISA kit; MGC129967; MGC129968; MGC138856; OCTD; ornithine transcarbamylase; ornithine carbamoyltransferase

other names :

ornithine carbamoyltransferase, mitochondrial; Ornithine carbamoyltransferase, mitochondrial; ornithine carbamoyltransferase, mitochondrial; OTCase; sparse fur; ornithine transcarbamylase; Ornithine transcarbamylase; OTCase

products gene name :

OTC

other gene names :

Otc; Otc; Sf; spf; AI265390; OTCase

uniprot entry name :

OTC_MOUSE

reactivity :

Mouse

sequence length :

354

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse OTC. No significant cross-reactivity or interference between mouse OTC and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 3.12 mU/ml -200 mU/ml. Sensitivity: The minimum detectable dose of mouse OTC is typically less than 0.78 mU/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for OTC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any OTC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for OTC is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of OTC bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

6679184

ncbi acc num :

NP_032795.1

ncbi gb acc num :

NM_008769.3

uniprot acc num :

P11725

ncbi mol weight :

39,765 Da

ncbi pathways :

Amino Acid Metabolism Pathway (198416); Arginine And Proline Metabolism Pathway (83157); Arginine And Proline Metabolism Pathway (323); Biosynthesis Of Amino Acids Pathway (790952); Biosynthesis Of Amino Acids Pathway (795174); Metabolic Pathways (132962); Metabolism Pathway (971233); Metabolism Of Amino Acids And Derivatives Pathway (970519); Urea Cycle Pathway (421751); Urea Cycle Pathway (970518)

uniprot summary :

OTC: Defects in OTC are the cause of ornithine carbamoyltransferase deficiency (OTCD). OTCD is an X- linked disorder of the urea cycle which causes a form of hyperammonemia. Mutations with no residual enzyme activity are always expressed in hemizygote males by a very severe neonatal hyperammonemic coma that generally proves to be fatal. Heterozygous females are either asymptomatic or express orotic aciduria spontaneously or after protein intake. The disorder is treatable with supplemental dietary arginine and low protein diet. The arbitrary classification of patients into the neonatal group (clinical hyperammonemia in the first few days of life) and late onset (clinical presentation after the neonatal period) has been used to differentiate severe from mild forms. Belongs to the ATCase/OTCase family. Protein type: Mitochondrial; Transferase; Amino Acid Metabolism - arginine and proline; EC 2.1.3.3. Cellular Component: mitochondrion; cytoplasm; mitochondrial inner membrane. Molecular Function: transferase activity; amino acid binding; carboxyl- or carbamoyltransferase activity; ornithine carbamoyltransferase activity; phospholipid binding; phosphate binding. Biological Process: amino acid metabolic process; citrulline biosynthetic process; arginine biosynthetic process; ornithine metabolic process; arginine biosynthetic process via ornithine; amino acid biosynthetic process; ornithine catabolic process; anion homeostasis; urea cycle

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage