Human PAS domain-containing serine/threonine-protein kinase, PASK ELISA Kit | MyBioSource MBS9326104 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human PAS domain-containing serine/threonine-protein kinase, PASK ELISA Kit

catalog :

MBS9326104

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS9326104

products type :

ELISA Kit

products full name :

Human PAS domain-containing serine/threonine-protein kinase, PASK ELISA Kit

products short name :

[PAS domain containing serine/threonine kinase]

products name syn :

[Human PAS domain-containing serine/threonine-protein kinase (PASK) ELISA kit; DKFZp434O051; DKFZp686P2031; KIAA0135; PASKIN; STK37; OTTHUMP00000200461; PAS-serine/threonine kinase; PAS domain containing serine/threonine kinase]

other names :

[PAS domain-containing serine/threonine-protein kinase isoform 1; PAS domain-containing serine/threonine-protein kinase; PAS domain-containing serine/threonine-protein kinase; per-arnt-sim (PAS) domain kinase; PAS domain containing serine/threonine kinase]

products gene name :

[PASK]

other gene names :

[PASK; PASK; STK37; PASKIN; KIAA0135; PAS-kinase; PASKIN; hPASK]

uniprot entry name :

PASK_HUMAN

reactivity :

Human

specificity :

No significant cross-reactivity or interference between this analyte and analogues is observed.

storage stability :

Store all reagents at 2-8 degree C

other info1 :

Samples: Undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances. Assay Type: Sandwich. Detection Range: 0.625 ng/ml - 20 ng/ml. Sensitivity: 0.1 ng/ml.

other info2 :

Intra-assay Precision: Intra-assay CV (%) is less than 15%. Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].

products description :

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, NOT for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of PASK (hereafter termed "analyte") in undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.

ncbi gi num :

355477262

ncbi acc num :

NP_001239048.1

ncbi gb acc num :

NM_001252119.1

uniprot acc num :

Q96RG2

ncbi mol weight :

142,929 Da

ncbi summary :

This gene encodes a member of the serine/threonine kinase family that contains two PAS domains. Expression of this gene is regulated by glucose, and the encoded protein plays a role in the regulation of insulin gene expression. Downregulation of this gene may play a role in type 2 diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]

uniprot summary :

PASK: Serine/threonine-protein kinase involved in energy homeostasis and protein translation. Phosphorylates EEF1A1, GYS1, PDX1 and RPS6. Probably plays a role under changing environmental conditions (oxygen, glucose, nutrition), rather than under standard conditions. Acts as a sensor involved in energy homeostasis: regulates glycogen synthase synthesis by mediating phosphorylation of GYS1, leading to GYS1 inactivation. May be involved in glucose-stimulated insulin production in pancreas and regulation of glucagon secretion by glucose in alpha cells; however such data require additional evidences. May play a role in regulation of protein translation by phosphorylating EEF1A1, leading to increase translation efficiency. May also participate to respiratory regulation. Ubiquitously expressed, with slightly higher expression in brain, prostate and testis. Reduced expression was found in placenta. Present in germ cells of testis and in the midpiece of sperm tails. Protein kinase activity is inhibited by the first PAS domain: binding of an unidentified ligand desinhibits the protein kinase activity. May be activated by autophosphorylation on Thr-1161 and Thr-1165 (PubMed:11459942). The activating role of autophosphorylation at Thr-1161 is unclear: according to a report, autophosphorylation at Thr-1161 does not play a major role in activation (PubMed:20943661). Autophosphorylation is enhanced upon phosphatidylinositol monophosphate (phosphatidylinositol 4-phosphate) binding and inhibited upon phosphatidylinositol bi- and tri-phosphate binding. In contrast, phosphorylation of target proteins is inhibited upon all phosphatidylinositol-binding (phosphatidylinositol mono- bi- and tri-phosphate). Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; Kinase, protein; EC 2.7.11.1; CAMK group; CAMKL family; PASK subfamily. Chromosomal Location of Human Ortholog: 2q37.3. Cellular Component: Golgi apparatus; cytoplasm; nucleus. Molecular Function: protein serine/threonine kinase activity; signal transducer activity; protein binding; phosphoinositide binding; ATP binding. Biological Process: regulation of respiratory gaseous exchange; positive regulation of translation; protein amino acid autophosphorylation; signal transduction; protein amino acid phosphorylation; negative regulation of glycogen biosynthetic process

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

680

size3 :

5x96-Strip-Wells

price3 :

3100

size4 :

10x96-Strip-Wells

price4 :

6095

more info or order :

MyBioSource product webpage