catalog number :
MBS932599
products type :
ELISA Kit
products full name :
Human CAMPATH-1 antigen, CD52 ELISA Kit
products short name :
[CD52 molecule]
products name syn :
[Human CAMPATH-1 antigen (CD52) ELISA kit; CDW52; CD52 antigen; CD52 antigen (CAMPATH-1 antigen) ; CDW52 antigen (CAMPATH-1 antigen) ; CD52 molecule]
other names :
[CAMPATH-1 antigen; CAMPATH-1 antigen; CAMPATH-1 antigen; he5; HEL-S-171mP; cambridge pathology 1 antigen; epididymal secretory protein E5; CD52 antigen (CAMPATH-1 antigen); CDW52 antigen (CAMPATH-1 antigen); human epididymis-specific protein 5; epididymis secretory sperm binding protein Li 171mP; CD52 molecule; CDw52; Cambridge pathology 1 antigen; Epididymal secretory protein E5; Human epididymis-specific protein 5; He5; CD_antigen: CD52]
products gene name :
[CD52]
other gene names :
[CD52; CD52; CDW52; CDW52; HE5; He5]
uniprot entry name :
CD52_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse CD47. No significant cross-reactivity or interference between mouse CD47 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Tissue Homogenates. Assay Type: Quantitative Sandwich. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: 3.9 pg/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CD47 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD47 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD47 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD47 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001794.2
ncbi gb acc num :
NM_001803.2
ncbi mol weight :
6,614 Da
uniprot summary :
CD52: May play a role in carrying and orienting carbohydrate, as well as having a more specific role. Protein type: Membrane protein, integral; Membrane protein, GPI anchor. Chromosomal Location of Human Ortholog: 1p36. Cellular Component: membrane; integral to plasma membrane. Biological Process: respiratory burst; elevation of cytosolic calcium ion concentration
size1 :
24-Strip-Wells (LIMIT 1)
size5 :
10x96-Strip-Wells